RRC ID 43796
Author Kok YY, Mooi LY, Ahmad K, Sukari MA, Mat N, Rahmani M, Ali AM.
Title Anti-tumour promoting activity and antioxidant properties of girinimbine isolated from the stem bark of Murraya koenigii S.
Journal Molecules
Abstract Girinimbine, a carbazole alkaloid isolated from the stem bark of Murraya koenigii was tested for the in vitro anti-tumour promoting and antioxidant activities. Anti-tumour promoting activity was determined by assaying the capability of this compound to inhibit the expression of early antigen of Epstein-Barr virus (EA-EBV) in Raji cells that was induced by the tumour promoter, phorbol 12-myristate 13-acetate. The concentration of this compound that gave an inhibition rate at fifty percent was 6.0 µg/mL and was not cytotoxic to the cells. Immunoblotting analysis of the expression of EA-EBV showed that girinimbine was able to suppress restricted early antigen (EA-R). However, diffused early antigen (EA-D) was partially suppressed when used at 32.0 µg/mL. Girinimbine exhibited a very strong antioxidant activity as compared to a-tocopherol and was able to inhibit superoxide generation in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells more than 95%, when treated with the compound at 5.3 and 26.3 µg/mL, respectively. However girinimbine failed to scavenge the stable diphenyl picryl hydrazyl (DPPH)-free radical.
Volume 17(4)
Pages 4651-60
Published 2012-4-20
DOI 10.3390/molecules17044651
PII molecules17044651
PMID 22522395
PMC PMC6268782
MeSH Alkaloids / isolation & purification Alkaloids / pharmacology* Antigens, Viral / genetics Antineoplastic Agents, Phytogenic / isolation & purification Antineoplastic Agents, Phytogenic / pharmacology* Antioxidants / isolation & purification Antioxidants / pharmacology* Cell Line, Tumor Cell Survival / drug effects Gene Expression / drug effects Humans Murraya / chemistry* Plant Bark / chemistry* Plant Stems / chemistry* Superoxides / metabolism
IF 3.06
Times Cited 19
Human and Animal Cells