| RRC ID |
43796
|
| 著者 |
Kok YY, Mooi LY, Ahmad K, Sukari MA, Mat N, Rahmani M, Ali AM.
|
| タイトル |
Anti-tumour promoting activity and antioxidant properties of girinimbine isolated from the stem bark of Murraya koenigii S.
|
| ジャーナル |
Molecules
|
| Abstract |
Girinimbine, a carbazole alkaloid isolated from the stem bark of Murraya koenigii was tested for the in vitro anti-tumour promoting and antioxidant activities. Anti-tumour promoting activity was determined by assaying the capability of this compound to inhibit the expression of early antigen of Epstein-Barr virus (EA-EBV) in Raji cells that was induced by the tumour promoter, phorbol 12-myristate 13-acetate. The concentration of this compound that gave an inhibition rate at fifty percent was 6.0 µg/mL and was not cytotoxic to the cells. Immunoblotting analysis of the expression of EA-EBV showed that girinimbine was able to suppress restricted early antigen (EA-R). However, diffused early antigen (EA-D) was partially suppressed when used at 32.0 µg/mL. Girinimbine exhibited a very strong antioxidant activity as compared to a-tocopherol and was able to inhibit superoxide generation in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells more than 95%, when treated with the compound at 5.3 and 26.3 µg/mL, respectively. However girinimbine failed to scavenge the stable diphenyl picryl hydrazyl (DPPH)-free radical.
|
| 巻・号 |
17(4)
|
| ページ |
4651-60
|
| 公開日 |
2012-4-20
|
| DOI |
10.3390/molecules17044651
|
| PII |
molecules17044651
|
| PMID |
22522395
|
| PMC |
PMC6268782
|
| MeSH |
Alkaloids / isolation & purification
Alkaloids / pharmacology*
Antigens, Viral / genetics
Antineoplastic Agents, Phytogenic / isolation & purification
Antineoplastic Agents, Phytogenic / pharmacology*
Antioxidants / isolation & purification
Antioxidants / pharmacology*
Cell Line, Tumor
Cell Survival / drug effects
Gene Expression / drug effects
Humans
Murraya / chemistry*
Plant Bark / chemistry*
Plant Stems / chemistry*
Superoxides / metabolism
|
| IF |
3.267
|
| 引用数 |
19
|
|
WOS 分野
|
CHEMISTRY, ORGANIC
|
| リソース情報 |
| ヒト・動物細胞 |
|
