RRC ID 43806
Author Okamoto M, Kobayashi S, Ikeuchi H, Yamada S, Yamanouchi K, Nagasawa K, Maekawa S, Kato T, Shimizu I.
Title Synthesis and bioassay of a boron-dipyrromethene derivative of estradiol for fluorescence imaging in vivo.
Journal Steroids
Abstract C7α-substituted estradiols bind to estrogen receptors in cell nuclei, yet these derivatives remain little used in bioimaging. Here, we describe a fluorescent derivative of estradiol (E2) with a boron-dipyrromethene (BODIPY) moiety attached to C7α, synthesized by olefin metathesis reaction of 7α-allylestradiol and 9-decenyl-BODIPY. In ovariectomized rats and non-ovariectomized mice, E2-BODIPY promoted the growth of uterine tissue similar to the effect of estradiol. Twenty-four hours after subcutaneous injection of E2-BODIPY in non-ovariectomized mice, we observed fluorescence of E2-BODIPY in the nuclei of uterine epithelial cells. Our findings suggest that fluorescence microscopy can localize this derivative in E2-responsive cells during normal development and tumorigenesis in vivo.
Volume 77(8-9)
Pages 845-9
Published 2012-7-1
DOI 10.1016/j.steroids.2012.04.011
PII S0039-128X(12)00137-7
PMID 22542503
MeSH Animals Boron Compounds / chemistry* Estradiol / chemistry* Female Mice Microscopy, Fluorescence / methods* Ovariectomy Rats Uterus / metabolism
IF 1.948
Times Cited 13
Human and Animal Cells