| RRC ID |
43806
|
| 著者 |
Okamoto M, Kobayashi S, Ikeuchi H, Yamada S, Yamanouchi K, Nagasawa K, Maekawa S, Kato T, Shimizu I.
|
| タイトル |
Synthesis and bioassay of a boron-dipyrromethene derivative of estradiol for fluorescence imaging in vivo.
|
| ジャーナル |
Steroids
|
| Abstract |
C7α-substituted estradiols bind to estrogen receptors in cell nuclei, yet these derivatives remain little used in bioimaging. Here, we describe a fluorescent derivative of estradiol (E2) with a boron-dipyrromethene (BODIPY) moiety attached to C7α, synthesized by olefin metathesis reaction of 7α-allylestradiol and 9-decenyl-BODIPY. In ovariectomized rats and non-ovariectomized mice, E2-BODIPY promoted the growth of uterine tissue similar to the effect of estradiol. Twenty-four hours after subcutaneous injection of E2-BODIPY in non-ovariectomized mice, we observed fluorescence of E2-BODIPY in the nuclei of uterine epithelial cells. Our findings suggest that fluorescence microscopy can localize this derivative in E2-responsive cells during normal development and tumorigenesis in vivo.
|
| 巻・号 |
77(8-9)
|
| ページ |
845-9
|
| 公開日 |
2012-7-1
|
| DOI |
10.1016/j.steroids.2012.04.011
|
| PII |
S0039-128X(12)00137-7
|
| PMID |
22542503
|
| MeSH |
Animals
Boron Compounds / chemistry*
Estradiol / chemistry*
Female
Mice
Microscopy, Fluorescence / methods*
Ovariectomy
Rats
Uterus / metabolism
|
| IF |
1.948
|
| 引用数 |
13
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
