| RRC ID |
43822
|
| 著者 |
Ohgidani M, Komizu Y, Goto K, Ueoka R.
|
| タイトル |
Residual powders from Shochu distillation remnants induce apoptosis in human hepatoma cells via the caspase-independent pathway.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Shochu distillation remnants (SDR) are by-products in the manufacturing process of the Japanese liquor Shochu and include various useful organic compounds derived from the fermentation of grains. We have obtained valuable powder (PSDR) from freeze-dried SDR by the treatment with ethanol. In this study, we examined the anticancer effects of barley-, rice-, and sweet potato-PSDR against HepG2 and HuH-7 cells of human hepatocellular carcinoma (HCC) in vitro. All PSDR inhibited the growth of both these HCC cells through the induction of apoptosis. Especially, barley-PSDR was the most effective for the growth inhibition and apoptosis induction of HCC cells of all PSDR. We next examined the apoptotic mechanisms induced by barley-PSDR. Decrease in mitochondrial membrane potential and release of cytochrome c from mitochondria were observed in HCC cells after the treatment with barley-PSDR. Furthermore, barley-PSDR induced the nuclear translocation of apoptosis-inducing factor (AIF) from mitochondria, while it did not significantly affect the activities of caspase-3, -8, and -9. The results suggested that barley-PSDR induced apoptosis against HCC cells via the caspase-independent mitochondrial pathway. The findings in this study suggest that PSDR has the possibility of therapeutic and/or preventive agents of HCC.
|
| 巻・号 |
114(1)
|
| ページ |
104-9
|
| 公開日 |
2012-7-1
|
| DOI |
10.1016/j.jbiosc.2012.02.026
|
| PII |
S1389-1723(12)00087-4
|
| PMID |
22560085
|
| MeSH |
Active Transport, Cell Nucleus
Apoptosis / drug effects*
Apoptosis Inducing Factor / metabolism
Carcinoma, Hepatocellular / drug therapy
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / pathology*
Caspases / metabolism*
Cell Line, Tumor
Cell Nucleus / metabolism
Cell Proliferation / drug effects
Cytochromes c / metabolism
Distillation*
Hep G2 Cells
Humans
Liver Neoplasms / metabolism
Liver Neoplasms / pathology*
Membrane Potential, Mitochondrial / drug effects
Mitochondria / drug effects
Powders / chemical synthesis
Powders / metabolism
Powders / pharmacology*
Powders / therapeutic use
|
| IF |
2.366
|
| 引用数 |
5
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2
HuH-7 |
