| RRC ID |
43839
|
| Author |
Tahara Y, Kaneko T, Toita R, Yoshiyama C, Kitaoka T, Niidome T, Katayama Y, Kamiya N, Goto M.
|
| Title |
A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells.
|
| Journal |
J Control Release
|
| Abstract |
A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.
|
| Volume |
161(3)
|
| Pages |
713-21
|
| Published |
2012-8-10
|
| DOI |
10.1016/j.jconrel.2012.05.001
|
| PII |
S0168-3659(12)00329-X
|
| PMID |
22580226
|
| MeSH |
Animals
CHO Cells
Cholesterol / chemistry
Cricetinae
Cricetulus
Cytochromes c / administration & dosage*
DNA / administration & dosage
Drug Carriers / administration & dosage*
Drug Carriers / chemistry
Horseradish Peroxidase / administration & dosage*
Nanotechnology / methods
Oils / chemistry
Oligopeptides / chemistry
Ovalbumin / administration & dosage*
Particle Size
Phosphatidylcholines / chemistry
Phosphatidylethanolamines / chemistry
Stearates / chemistry
Transfection / methods*
Water / chemistry
|
| IF |
7.727
|
| Times Cited |
8
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
|
