RRC ID 4384
Author Poch HL, López RH, Clark SJ.
Title Ecotypes of the model legume Lotus japonicus vary in their interaction phenotypes with the root-knot nematode Meloidogyne incognita.
Journal Ann. Bot.
Abstract BACKGROUND AND AIMS:Knowledge of host factors affecting plant-nematode interactions is scarce. Here, relevant interaction phenotypes between a nodulating model host, Lotus japonicus, and the endoparasitic root-knot nematode Meloidogyne incognita are assessed via a genetic screen.
METHODS:Within an alpha experimental design, 4-week-old replicate plants from 60 L. japonicus ecotypes were inoculated with 1000 nematodes from a single egg mass population, and evaluated for galling and nematode egg masses 6 weeks after inoculation.
KEY RESULTS:Statistical analysis of data for 57 ecotypes showed that ecotype susceptibilities ranged from 3.5 to 406 galls per root, and correlated strongly (r = 0.8, P < 0.001, log scale) with nematode reproduction (ranging from 0.6 to 34.5 egg masses per root). Some ecotypes, however, showed a significant discrepancy between disease severity and nematode reproduction. Necrosis and developmental malformations were observed in other infected ecotypes.
CONCLUSIONS:The first evidence is provided of significant variability in the interactions between L. japonicus and root-knot nematodes that may have further implications for the genetic dissection and characterization of host pathways involved in nematode parasitism and, possibly, in microbial symbiosis.
Volume 99(6)
Pages 1223-9
Published 2007-6
DOI 10.1093/aob/mcm058
PII mcm058
PMID 17475628
PMC PMC3243576
MeSH Animals Host-Parasite Interactions / physiology Lotus / classification Lotus / growth & development* Lotus / parasitology Nematoda / growth & development* Plant Diseases / parasitology Plant Roots / growth & development* Plant Roots / parasitology Species Specificity
IF 3.454
Times Cited 8
Lotus / Glycine Miyakojima MG-20