論文 - 詳細
| RRC ID | 43841 |
|---|---|
| 著者 | Hahne M, Zorn-Kruppa M, Guzman G, Brandner JM, Haltner-Ukomado E, Wätzig H, Reichl S. |
| タイトル | Prevalidation of a human cornea construct as an alternative to animal corneas for in vitro drug absorption studies. |
| ジャーナル | J Pharm Sci |
| Abstract |
The use of ophthalmic drugs has increased consistently over the past few decades. Currently, most research is conducted using in vivo and ex vivo animal experiments; however, they have many disadvantages, including ethical concerns, high costs, the questionable extension of animal results to humans, and poor standardization. Although several cell culture-based cornea models have been developed, none have been validated and accepted for general use. In this study, a standardized, three-dimensional model of the human cornea (Hemicornea, HC) based on immortalized human corneal cells and cultivated in serum-free conditions was developed for drug absorption studies and prevalidated using compounds with a wide range of molecular characteristics (sodium fluorescein, rhodamine B, fluorescein isothiocyanate-labeled dextran, aciclovir, bimatoprost, dexamethasone, and timolol maleate). The HC model was independently cultured in three different laboratories, and the intralaboratory and interlaboratory reproducibility was analyzed and compared with the rabbit cornea. This analysis showed that the HC has a barrier in the same range as excised animal corneas, although with a higher reproducibility and lower variability. Because of the demonstrated transferability, the HC represents a promising in vitro alternative to the use of ex vivo tissue and offers a well-defined and standardized system for drug absorption studies. |
| 巻・号 | 101(8) |
| ページ | 2976-88 |
| 公開日 | 2012-8-1 |
| DOI | 10.1002/jps.23190 |
| PII | S0022-3549(15)31481-7 |
| PMID | 22581751 |
| MeSH | Administration, Ophthalmic Animals Cell Culture Techniques Cell Line Epithelium, Corneal / cytology* Epithelium, Corneal / metabolism* Epithelium, Corneal / ultrastructure Female Humans Ophthalmic Solutions / administration & dosage* Pharmaceutical Preparations / administration & dosage* Pharmacokinetics Rabbits Swine |
| IF | 2.997 |
| 引用数 | 24 |
| WOS 分野 | CHEMISTRY, MEDICINAL PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |