RRC ID 43859
Author Aravind A, Jeyamohan P, Nair R, Veeranarayanan S, Nagaoka Y, Yoshida Y, Maekawa T, Kumar DS.
Title AS1411 aptamer tagged PLGA-lecithin-PEG nanoparticles for tumor cell targeting and drug delivery.
Journal Biotechnol Bioeng
Abstract Liposomes and polymers are widely used drug carriers for controlled release since they offer many advantages like increased treatment effectiveness, reduced toxicity and are of biodegradable nature. In this work, anticancer drug-loaded PLGA-lecithin-PEG nanoparticles (NPs) were synthesized and were functionalized with AS1411 anti-nucleolin aptamers for site-specific targeting against tumor cells which over expresses nucleolin receptors. The particles were characterized by transmission electron microscope (TEM) and X-ray photoelectron spectroscopy (XPS). The drug-loading efficiency, encapsulation efficiency and in vitro drug release studies were conducted using UV spectroscopy. Cytotoxicity studies were carried out in two different cancer cell lines, MCF-7 and GI-1 cells and two different normal cells, L929 cells and HMEC cells. Confocal microscopy and flowcytometry confirmed the cellular uptake of particles and targeted drug delivery. The morphology analysis of the NPs proved that the particles were smooth and spherical in shape with a size ranging from 60 to 110 nm. Drug-loading studies indicated that under the same drug loading, the aptamer-targeted NPs show enhanced cancer killing effect compared to the corresponding non-targeted NPs. In addition, the PLGA-lecithin-PEG NPs exhibited high encapsulation efficiency and superior sustained drug release than the drug loaded in plain PLGA NPs. The results confirmed that AS1411 aptamer-PLGA-lecithin-PEG NPs are potential carrier candidates for differential targeted drug delivery.
Volume 109(11)
Pages 2920-31
Published 2012-11-1
DOI 10.1002/bit.24558
PMID 22615073
MeSH Antineoplastic Agents / pharmacokinetics* Aptamers, Nucleotide Cell Line Cell Survival / drug effects Drug Carriers / chemistry Drug Carriers / pharmacokinetics* Flow Cytometry Humans Lactic Acid / chemistry Lactic Acid / pharmacokinetics* Lecithins / chemistry Lecithins / pharmacokinetics* Microscopy, Confocal Microscopy, Electron, Transmission Nanoparticles / chemistry* Oligodeoxyribonucleotides / chemistry Oligodeoxyribonucleotides / pharmacokinetics* Photoelectron Spectroscopy Polyethylene Glycols / chemistry Polyethylene Glycols / pharmacokinetics* Polyglycolic Acid / chemistry Polyglycolic Acid / pharmacokinetics* Polylactic Acid-Polyglycolic Acid Copolymer Spectrophotometry, Ultraviolet
IF 4.002
Times Cited 114
Human and Animal Cells