| RRC ID |
43860
|
| 著者 |
Tsuchiya K, Nitta N, Sonoda A, Nitta-Seko A, Ohta S, Takahashi M, Murata K, Mukaisho K, Shiomi M, Tabata Y, Nohara S.
|
| タイトル |
Evaluation of atherosclerotic lesions using dextran- and mannan-dextran-coated USPIO: MRI analysis and pathological findings.
|
| ジャーナル |
Int J Nanomedicine
|
| Abstract |
Magnetic resonance imaging (MRI) can detect atherosclerotic lesions containing accumulations of ultrasmall superparamagnetic iron oxides (USPIO). Positing that improved USPIO with a higher affinity for atherosclerotic plaques would yield better plaque images, we performed MRI and histologic studies to compare the uptake of dextran- and mannan-dextran-coated USPIO (D-USPIO and DM-USPIO, respectively) by the atherosclerotic walls of rabbits. We intravenously injected atherosclerotic rabbits with DM-USPIO (n = 5) or D-USPIO (n = 5). Two rabbits were the controls. The doses delivered were 0.08 (dose 1) (n = 1), 0.4 (dose 2) (n = 1), or 0.8 (dose 3) (n = 3) mmol iron/Kg. The dose 3 rabbits underwent in vivo contrast-enhanced magnetic resonance angiography (MRA) before and 5 days after USPIO administration. Afterwards, all animals were euthanized, the aortae were removed and subjected to in vitro MRI study. The signal-to-noise ratio (SNR) of the aortic wall in the same region of interest (ROI) was calculated in both in vivo and in vitro studies. Histological assessment through measurement of iron-positive regions in Prussian blue-stained specimens showed that iron-positive regions were significantly larger in rabbits injected with DM- rather than D-USPIO (P < 0.05) for all doses. In vivo MRA showed that the SNR-reducing effect of DM- was greater than that of D-USPIO (P < 0.05). With in vitro MRI scans, SNR was significantly lower in rabbits treated with dose 2 of DM-USPIO compared with D-USPIO treatment (P < 0.05), and it tended to be lower at dose 3 (P < 0.1). In conclusion, we suggest that DM-USPIO is superior to D-USPIO for the study of atherosclerotic lesions in rabbits.
|
| 巻・号 |
7
|
| ページ |
2271-80
|
| 公開日 |
2012-1-1
|
| DOI |
10.2147/IJN.S29417
|
| PII |
ijn-7-2271
|
| PMID |
22619561
|
| PMC |
PMC3356181
|
| MeSH |
Animals
Atherosclerosis / diagnosis*
Atherosclerosis / pathology
Cell Line
Coated Materials, Biocompatible
Dextrans
Hyperlipoproteinemia Type II / pathology
Macrophages / physiology
Magnetic Resonance Angiography / methods*
Magnetic Resonance Angiography / statistics & numerical data
Magnetite Nanoparticles*
Mannans
Mice
Nanomedicine
Phagocytosis
Rabbits
Signal-To-Noise Ratio
|
| IF |
5.115
|
| 引用数 |
8
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
J774.1(RCB0434) |
