Reference - Detail
| RRC ID | 43863 |
|---|---|
| Author | Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Yoshikawa M. |
| Title | In vitro differentiation of mouse embryonic stem cells into inner ear hair cell-like cells using stromal cell conditioned medium. |
| Journal | Cell Death Dis |
| Abstract |
Hearing loss is mainly caused by loss of sensory hair cells (HCs) in the organ of Corti or cochlea. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about the efficient generation of HC-like cells from ES cells. In the present study, we developed a single-medium culture method for growing embryoid bodies (EBs), in which conditioned medium (CM) from cultures of ST2 stromal cells (ST2-CM) was used for 14-day cultures of 4-day EBs. At the end of the 14-day cultures, up to 20% of the cells in EB outgrowths expressed HC-related markers, including Math1 (also known as Atoh1), myosin6, myosin7a, calretinin, α9AchR and Brn3c (also known as Pou4f3), and also showed formation of stereocilia-like structures. Further, we found that these cells were incorporated into the developing inner ear after transplantation into chick embryos. The present inner ear HC induction method using ST2-CM (HIST2 method) is quite simple and highly efficient to obtain ES-derived HC-like cells with a relatively short cultivation time. |
| Volume | 3(5) |
| Pages | e314 |
| Published | 2012-5-24 |
| DOI | 10.1038/cddis.2012.56 |
| PII | cddis201256 |
| PMID | 22622133 |
| PMC | PMC3366087 |
| MeSH | Animals Cell Differentiation* Chick Embryo Culture Media, Conditioned / metabolism Embryoid Bodies / cytology Embryoid Bodies / metabolism Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Hair Cells, Auditory, Inner / cytology* Hair Cells, Auditory, Inner / metabolism Mice Stromal Cells / cytology Stromal Cells / metabolism |
| IF | 6.304 |
| Times Cited | 20 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ST2(RCB0224) MC3T3-G2/PA6(RCB1127) |