Reference - Detail
| RRC ID | 43896 |
|---|---|
| Author | Adfa M, Itoh T, Hattori Y, Koketsu M. |
| Title | Inhibitory effects of 6-alkoxycoumarin and 7-alkoxycoumarin derivatives on lipopolysaccharide/interferon γ-stimulated nitric oxide production in RAW264 cells. |
| Journal | Biol Pharm Bull |
| Abstract |
Coumarin and its derivatives are well known for their anti-inflammatory and anti-oxidative effects. In this study, we synthesized 32 coumarin derivatives from commercially available 6-hydroxycoumarin (6HC) and 7-hydroxycoumarin (7HC) and examined their effects on lipopolysaccharide/interferon γ (LPS/IFNγ)-stimulated nitric oxide (NO) production in murine macrophage RAW264 cells. Among these derivatives, 6HC-8 (6-(3-phenylpropoxy)coumarin), 6HC-14 (6-(2-octynyloxy)coumarin), 7HC-14 (7-(2-octynyloxy)coumarin), and 7HC-16 (7-(3,5-dimethoxybenzyloxy)coumarin) markedly suppressed NO production at low concentration (25 µM). These synthesized coumarin derivatives also markedly inhibited inducible NO synthase (iNOS) protein and mRNA expression, as assessed by western blotting and quantitative real time-polymerase chain reaction (RT-PCR). |
| Volume | 35(6) |
| Pages | 963-6 |
| Published | 2012-1-1 |
| DOI | 10.1248/bpb.35.963 |
| PII | DN/JST.JSTAGE/bpb/35.963 |
| PMID | 22687539 |
| MeSH | Animals Cell Line Coumarins / pharmacology* Interferon-gamma / pharmacology Lipopolysaccharides / pharmacology Mice Nitric Oxide / antagonists & inhibitors* Nitric Oxide / metabolism Nitric Oxide Synthase Type II / antagonists & inhibitors* Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism RNA, Messenger / metabolism |
| IF | 1.863 |
| Times Cited | 6 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |