RRC ID 4391
Author Ikeda R, Saito F, Matsuo M, Kurokawa K, Sekimizu K, Yamaguchi M, Kawamoto S.
Title Contribution of the mannan backbone of cryptococcal glucuronoxylomannan and a glycolytic enzyme of Staphylococcus aureus to contact-mediated killing of Cryptococcus neoformans.
Journal J. Bacteriol.
Abstract 3) oligosaccharides were predicted. The three-dimensional structures of mannooligosaccharides larger than triose appeared curved and could be imagined to be recognized by a hypothetical staphylococcal lectin. Native polyacrylamide gel electrophoresis of staphylococcal protein followed by electroblotting, enzyme-linked immunolectin assay, protein staining, and N-terminal amino acid sequencing suggested that the candidate protein was triosephosphate isomerase (TPI). The enzymatic activities were confirmed by using whole cells of S. aureus. TPI point mutants of S. aureus decreased the ability to interact with C. neoformans. Thus, TPI on S. aureus adheres to the capsule of C. neoformans by recognizing the structure of mannotriose units in the backbone of GXM; we suggest that this contact is required for killing of C. neoformans.
Volume 189(13)
Pages 4815-26
Published 2007-7
DOI 10.1128/JB.00412-07
PII JB.00412-07
PMID 17483230
PMC PMC1913461
MeSH Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism* Carbohydrate Conformation Cryptococcus neoformans / drug effects Cryptococcus neoformans / metabolism* Cryptococcus neoformans / ultrastructure Enzyme-Linked Immunosorbent Assay Glucuronic Acid / metabolism Mannans / chemistry Mannans / metabolism* Microscopy, Electron, Scanning Microscopy, Electron, Transmission Molecular Sequence Data Peptide Hydrolases / genetics Peptide Hydrolases / metabolism Periodic Acid / pharmacology Point Mutation Polysaccharides / chemistry Polysaccharides / metabolism* Staphylococcus aureus / enzymology Staphylococcus aureus / genetics Surface Plasmon Resonance Triose-Phosphate Isomerase / genetics Triose-Phosphate Isomerase / metabolism* Xylose / metabolism
IF 3.219
Times Cited 21
Pathogenic microorganisms IFM 5839 IFM 5840 IFM 5844 IFM 48642 IFM 48643