Reference - Detail
| RRC ID | 43912 |
|---|---|
| Author | Hasebe A, Nakamura Y, Tashima H, Takahashi K, Iijima M, Yoshimoto N, Ting K, Kuroda S, Niimi T. |
| Title | The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1. |
| Journal | FEBS Lett |
| Abstract |
NELL1 is a secretory osteogenic protein containing several structural motifs that suggest that it functions as an extracellular matrix component. To determine the mechanisms underlying NELL1-induced osteoblast differentiation, we examined the cell-adhesive activity of NELL1 using a series of recombinant NELL1 proteins. We demonstrated that NELL1 promoted osteoblastic cell adhesion through at least three cell-binding domains located in the C-terminal region of NELL1. Adhesion of cells to NELL1 was strongly inhibited by function-blocking antibodies against integrin α3 and β1 subunits, suggesting that osteoblastic cells adhered to NELL1 through integrin α3β1. Further, focal adhesion kinase activation is involved in NELL1 signaling. |
| Volume | 586(16) |
| Pages | 2500-6 |
| Published | 2012-7-30 |
| DOI | 10.1016/j.febslet.2012.06.014 |
| PII | S0014-5793(12)00492-9 |
| PMID | 22728432 |
| MeSH | 3T3 Cells Animals Calcium-Binding Proteins / chemistry* Calcium-Binding Proteins / physiology Cell Adhesion Cell Line, Tumor Dose-Response Relationship, Drug Gene Expression Regulation* Glycoproteins / chemistry* Glycoproteins / physiology Humans Integrin alpha3beta1 / biosynthesis* MAP Kinase Signaling System Mice Mice, Inbred C3H Models, Biological Osteoblasts / cytology* Plasmids / metabolism Protein Structure, Tertiary Recombinant Proteins / chemistry Signal Transduction |
| IF | 3.057 |
| Times Cited | 15 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |