論文 - 詳細
| RRC ID | 43914 |
|---|---|
| 著者 | Zhang K, Yamamoto Y, Suzuki T, Yokota K, Ma S, Ni Nengah Dwi Fatmawati, Oguma K. |
| タイトル | Clostridium botulinum type E toxins bind to Caco-2 cells by a different mechanism from that of type A toxins. |
| ジャーナル | Acta Med Okayama |
| Abstract |
Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin. |
| 巻・号 | 66(3) |
| ページ | 253-61 |
| 公開日 | 2012-1-1 |
| DOI | 10.18926/AMO/48565 |
| PMID | 22729106 |
| MeSH | Blotting, Western Botulinum Toxins / isolation & purification Botulinum Toxins / metabolism* Botulinum Toxins, Type A / isolation & purification Botulinum Toxins, Type A / metabolism* Caco-2 Cells Humans |
| IF | 0.642 |
| 引用数 | 0 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |