RRC ID |
43919
|
著者 |
Sugihara Y, Taniguchi H, Kushima R, Tsuda H, Kubota D, Ichikawa H, Sakamoto K, Nakamura Y, Tomonaga T, Fujita S, Kondo T.
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タイトル |
Proteomic-based identification of the APC-binding protein EB1 as a candidate of novel tissue biomarker and therapeutic target for colorectal cancer.
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ジャーナル |
J Proteomics
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Abstract |
Novel candidates of biomarker and therapeutic target in colorectal cancer (CRC) were investigated using a proteomic approach. The proteome of normal colorectal epithelial tissues was compared with that of the tumor ones in 59 CRC patients using two-dimensional difference gel electrophoresis. Of 3458 protein spots, 110 exhibited statistically significant (p<0.01) differences in intensity (more than 2.5-folds) between the normal and tumor tissue groups. Of 67 unique gene products that were identified for 105 of the 110 protein spots, we focused on the higher expression of the adenoma polyposis coli-binding protein EB1 (EB1). EB1 was originally discovered as a binding protein of APC, which is a tumor suppressor gene product, and the expression of EB1 has been associated with poor prognosis in several malignancies but not in CRC. Immunohistochemical analysis of the 132 CRC cases revealed that EB1 was overexpressed in tumor cells in correlation with poor prognosis. Suppression of EB1 by RNAi inhibited CRC cell proliferation and invasion. In this study, the overexpression of EB1 in CRC tissues correlating with prognosis, and its functional contribution to the malignant phenotypes of CRC cells are described. The present findings indicate that EB1 is a potential biomarker and therapeutic target in CRC.
|
巻・号 |
75(17)
|
ページ |
5342-55
|
公開日 |
2012-9-18
|
DOI |
10.1016/j.jprot.2012.06.013
|
PII |
S1874-3919(12)00456-3
|
PMID |
22735596
|
MeSH |
Adenocarcinoma / diagnosis
Adenocarcinoma / drug therapy
Adenocarcinoma / metabolism*
Adenocarcinoma / pathology
Adenomatous Polyposis Coli Protein / metabolism
Aged
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / therapeutic use
Biomarkers, Tumor / analysis
Biomarkers, Tumor / antagonists & inhibitors
Biomarkers, Tumor / metabolism
Biomarkers, Tumor / physiology*
Colorectal Neoplasms / diagnosis
Colorectal Neoplasms / drug therapy
Colorectal Neoplasms / metabolism*
Colorectal Neoplasms / pathology
Drug Discovery
Female
Humans
Male
Microtubule-Associated Proteins / analysis
Microtubule-Associated Proteins / antagonists & inhibitors
Microtubule-Associated Proteins / metabolism
Microtubule-Associated Proteins / physiology*
Middle Aged
Molecular Targeted Therapy* / methods
Neoplasm Proteins / analysis
Neoplasm Proteins / metabolism
Neoplasm Proteins / physiology
Protein Binding
Proteomics / methods
|
IF |
3.509
|
引用数 |
22
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
リソース情報 |
ヒト・動物細胞 |
|