RRC ID 43919
著者 Sugihara Y, Taniguchi H, Kushima R, Tsuda H, Kubota D, Ichikawa H, Sakamoto K, Nakamura Y, Tomonaga T, Fujita S, Kondo T.
タイトル Proteomic-based identification of the APC-binding protein EB1 as a candidate of novel tissue biomarker and therapeutic target for colorectal cancer.
ジャーナル J Proteomics
Abstract Novel candidates of biomarker and therapeutic target in colorectal cancer (CRC) were investigated using a proteomic approach. The proteome of normal colorectal epithelial tissues was compared with that of the tumor ones in 59 CRC patients using two-dimensional difference gel electrophoresis. Of 3458 protein spots, 110 exhibited statistically significant (p<0.01) differences in intensity (more than 2.5-folds) between the normal and tumor tissue groups. Of 67 unique gene products that were identified for 105 of the 110 protein spots, we focused on the higher expression of the adenoma polyposis coli-binding protein EB1 (EB1). EB1 was originally discovered as a binding protein of APC, which is a tumor suppressor gene product, and the expression of EB1 has been associated with poor prognosis in several malignancies but not in CRC. Immunohistochemical analysis of the 132 CRC cases revealed that EB1 was overexpressed in tumor cells in correlation with poor prognosis. Suppression of EB1 by RNAi inhibited CRC cell proliferation and invasion. In this study, the overexpression of EB1 in CRC tissues correlating with prognosis, and its functional contribution to the malignant phenotypes of CRC cells are described. The present findings indicate that EB1 is a potential biomarker and therapeutic target in CRC.
巻・号 75(17)
ページ 5342-55
公開日 2012-9-18
DOI 10.1016/j.jprot.2012.06.013
PII S1874-3919(12)00456-3
PMID 22735596
MeSH Adenocarcinoma / diagnosis Adenocarcinoma / drug therapy Adenocarcinoma / metabolism* Adenocarcinoma / pathology Adenomatous Polyposis Coli Protein / metabolism Aged Antineoplastic Agents / isolation & purification Antineoplastic Agents / therapeutic use Biomarkers, Tumor / analysis Biomarkers, Tumor / antagonists & inhibitors Biomarkers, Tumor / metabolism Biomarkers, Tumor / physiology* Colorectal Neoplasms / diagnosis Colorectal Neoplasms / drug therapy Colorectal Neoplasms / metabolism* Colorectal Neoplasms / pathology Drug Discovery Female Humans Male Microtubule-Associated Proteins / analysis Microtubule-Associated Proteins / antagonists & inhibitors Microtubule-Associated Proteins / metabolism Microtubule-Associated Proteins / physiology* Middle Aged Molecular Targeted Therapy* / methods Neoplasm Proteins / analysis Neoplasm Proteins / metabolism Neoplasm Proteins / physiology Protein Binding Proteomics / methods
IF 3.509
引用数 22
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
ヒト・動物細胞