RRC ID 43922
著者 Morimoto S, Anada T, Honda Y, Suzuki O.
タイトル Comparative study on in vitro biocompatibility of synthetic octacalcium phosphate and calcium phosphate ceramics used clinically.
ジャーナル Biomed Mater
Abstract The present study was designed to investigate the extent to which calcium phosphate bone substitute materials, including osteoconductive octacalcium phosphate (OCP), display cytotoxic and inflammatory responses based on their dissolution in vitro. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ceramics, which are clinically used, as well as dicalcium phosphate dihydrate (DCPD) and synthesized OCP were compared. The materials were well characterized by chemical analysis, x-ray diffraction and Fourier transform infrared spectroscopy. Calcium and phosphate ion concentrations and the pH of culture media after immersion of the materials were determined. The colony forming rate of Chinese hamster lung fibroblasts was estimated with extraction of the materials. Proliferation of bone marrow stromal ST-2 cells and inflammatory cytokine TNF-α production by THP-1 cells grown on the material-coated plates were examined. The materials had characteristics that corresponded to those reported. DCPD was shown to dissolve the most in the culture media, with a marked increase in phosphate ion concentration and a reduction in pH. ST-2 cells proliferated well on the materials, with the exception of DCPD, which markedly inhibited cellular growth. The colony forming capacity was the lowest on DCPD, while that of the other calcium phosphates was not altered. In contrast, TNF-α was not detected even in cells grown on DCPD, suggesting that calcium phosphate materials are essentially non-inflammatory, while the solubility of the materials can affect osteoblastic and fibroblastic cellular attachment. These results indicate that OCP is biocompatible, which is similar to the materials used clinically, such as HA. Therefore, OCP could be clinically used as a biocompatible bone substitute material.
巻・号 7(4)
ページ 045020
公開日 2012-8-1
DOI 10.1088/1748-6041/7/4/045020
PMID 22740587
MeSH Animals Biocompatible Materials / chemistry* Bone Substitutes / chemistry* Calcium / chemistry Calcium Phosphates / chemistry* Cell Proliferation Ceramics / chemistry* Cricetinae Culture Media / chemistry Durapatite / chemistry Fibroblasts / cytology Humans Hydrogen-Ion Concentration In Vitro Techniques Inflammation Osteoblasts / cytology Spectroscopy, Fourier Transform Infrared / methods Tumor Necrosis Factor-alpha / metabolism X-Ray Diffraction
IF 3.174
引用数 22
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 ST2(RCB0224)