Reference - Detail
|Author||Sakaeyama S, Sano A, Murata Y, Kamei K, Nishimura K, Hatai K.|
|Title||Lecythophora hoffmannii isolated from a case of canine osteomyelitis in Japan.|
A 2-year-old spayed female mongrel dog showed claudication with abnormal ossification containing fungal cells detected by biopsy. The dog was treated with ketoconazole and itraconazole perorally for 5 months; however, the osteomyelitis became aggravated, and an amputation from the scapula was performed. The right superficial cervical lymph node became swollen 5 months after the operation. The lymph node contained PAS positive fungal elements and a portion of tissue produced mycelial fungal growth on potato dextrose agar supplemented with chloramphenicol. The culture was identified as Lecythophora hoffmannii based on morphology, physiology and 100% identity in the sequence of the D1/D2 domain of the large subunit ribosomal RNA gene of the fungal species in the GenBank database (accession number AB100627). In addition, the sequence from the present isolate was submitted as AB189164. The isolate showed resistance to antifungal agents, i.e., amphotericin B, 5-FC, fluconazole, itraconazole, miconazole and micafungin. The dog developed cachexia 2 months after the onset of lymphadenopathy, and was euthanized on the 459th day after onset of clinical symptoms. This was the first disseminated case of L. hoffmannii infection in Japan.
|MeSH||Animals Antifungal Agents / therapeutic use Ascomycota / cytology Ascomycota / genetics Ascomycota / isolation & purification* Ascomycota / physiology Biopsy DNA, Fungal / genetics DNA, Ribosomal / genetics Dog Diseases / drug therapy Dog Diseases / microbiology* Dogs Drug Resistance, Fungal Extremities / diagnostic imaging Extremities / microbiology Extremities / pathology Female Histocytochemistry Itraconazole / therapeutic use Japan Ketoconazole / therapeutic use Lymph Nodes / microbiology Lymph Nodes / pathology Microbial Sensitivity Tests Molecular Sequence Data Mycoses / complications Mycoses / drug therapy Mycoses / veterinary* Ossification, Heterotopic / diagnostic imaging Ossification, Heterotopic / veterinary Osteomyelitis / drug therapy Osteomyelitis / microbiology Osteomyelitis / veterinary* RNA, Ribosomal, 28S / genetics Radiography Sequence Analysis, DNA Sequence Homology, Nucleic Acid|
|WOS Category||VETERINARY SCIENCES MYCOLOGY INFECTIOUS DISEASES|
|Pathogenic microorganisms||IFM 4922 IFM 40213 IFM 51330 IFM 53859|