論文 - 詳細
| RRC ID | 43930 |
|---|---|
| 著者 | Cao M, Seike M, Soeno C, Mizutani H, Kitamura K, Minegishi Y, Noro R, Yoshimura A, Cai L, Gemma A. |
| タイトル | MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells. |
| ジャーナル | Int J Oncol |
| Abstract |
Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) has been shown to be related to the pathogenesis of various diseases including lung cancer. Recently, microRNAs (miRNA) have been recognized as a new class of genes involved in human tumorigenesis. MiR-23a/24/27a is a miRNA cluster located in chromosome 19p13.12, which can function as an oncogene in several human cancers. In this study, we analyzed miR-23a/24/27a expression in 10 non-small cell cancer (NSCLC) cell lines by real-time PCR analysis. Correlation between expression of these miRNAs and TGF-β/Smad signaling was evaluated. We found that miR-23a could be regulated by TGF-β1 in a Smad-dependent manner in A549 lung adenocarcinoma cells showing the EMT phenomenon. Knockdown of miR-23a partially restored E-cadherin expression under conditions of TGF-β1 stimulation. In contrast, overexpression of miR-23a could suppress E-cadherin expression and stimulate EMT. Furthermore, A549 cells with overexpressed miR-23a were more resistant to gefitinib compared to the parental cells. These findings suggest that miR-23a regulates TGF-β-induced EMT by targeting E-cadherin in lung cancer cells and may be useful as a new therapeutic target in NSCLC. |
| 巻・号 | 41(3) |
| ページ | 869-75 |
| 公開日 | 2012-9-1 |
| DOI | 10.3892/ijo.2012.1535 |
| PMID | 22752005 |
| PMC | PMC3582905 |
| MeSH | Cadherins / metabolism* Carcinoma, Non-Small-Cell Lung / metabolism Carcinoma, Non-Small-Cell Lung / physiopathology* Cell Line, Tumor Drug Resistance, Neoplasm Epithelial-Mesenchymal Transition* Gefitinib Humans Lung Neoplasms / metabolism Lung Neoplasms / physiopathology* MicroRNAs / biosynthesis MicroRNAs / genetics MicroRNAs / metabolism* Quinazolines / pharmacology RNA Interference RNA, Small Interfering Signal Transduction Transforming Growth Factor beta / metabolism* |
| IF | 3.899 |
| 引用数 | 123 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-KJ(RCB1313) LC-2/ad(RCB0440) |