| RRC ID |
43930
|
| 著者 |
Cao M, Seike M, Soeno C, Mizutani H, Kitamura K, Minegishi Y, Noro R, Yoshimura A, Cai L, Gemma A.
|
| タイトル |
MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) has been shown to be related to the pathogenesis of various diseases including lung cancer. Recently, microRNAs (miRNA) have been recognized as a new class of genes involved in human tumorigenesis. MiR-23a/24/27a is a miRNA cluster located in chromosome 19p13.12, which can function as an oncogene in several human cancers. In this study, we analyzed miR-23a/24/27a expression in 10 non-small cell cancer (NSCLC) cell lines by real-time PCR analysis. Correlation between expression of these miRNAs and TGF-β/Smad signaling was evaluated. We found that miR-23a could be regulated by TGF-β1 in a Smad-dependent manner in A549 lung adenocarcinoma cells showing the EMT phenomenon. Knockdown of miR-23a partially restored E-cadherin expression under conditions of TGF-β1 stimulation. In contrast, overexpression of miR-23a could suppress E-cadherin expression and stimulate EMT. Furthermore, A549 cells with overexpressed miR-23a were more resistant to gefitinib compared to the parental cells. These findings suggest that miR-23a regulates TGF-β-induced EMT by targeting E-cadherin in lung cancer cells and may be useful as a new therapeutic target in NSCLC.
|
| 巻・号 |
41(3)
|
| ページ |
869-75
|
| 公開日 |
2012-9-1
|
| DOI |
10.3892/ijo.2012.1535
|
| PMID |
22752005
|
| PMC |
PMC3582905
|
| MeSH |
Cadherins / metabolism*
Carcinoma, Non-Small-Cell Lung / metabolism
Carcinoma, Non-Small-Cell Lung / physiopathology*
Cell Line, Tumor
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition*
Gefitinib
Humans
Lung Neoplasms / metabolism
Lung Neoplasms / physiopathology*
MicroRNAs / biosynthesis
MicroRNAs / genetics
MicroRNAs / metabolism*
Quinazolines / pharmacology
RNA Interference
RNA, Small Interfering
Signal Transduction
Transforming Growth Factor beta / metabolism*
|
| IF |
3.899
|
| 引用数 |
123
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RERF-LC-KJ(RCB1313)
LC-2/ad(RCB0440) |
