論文 - 詳細
| RRC ID | 43945 |
|---|---|
| 著者 | Ramón-Azcón J, Ahadian S, Obregón R, Camci-Unal G, Ostrovidov S, Hosseini V, Kaji H, Ino K, Shiku H, Khademhosseini A, Matsue T. |
| タイトル | Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells. |
| ジャーナル | Lab Chip |
| Abstract |
Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e.g., proliferation, migration, differentiation, and apoptosis) and in making functional tissue constructs. One approach to spatially pattern cells is by dielectrophoresis (DEP). DEP has characteristics that are important for cell manipulation, such as high accuracy, speed, scalability, and the ability to handle both adherent and non-adherent cells. However, widespread application of this method is largely restricted because there is a limited number of suitable hydrogels for cell encapsulation. To date, polyethylene glycol-diacrylate (PEG-DA) and agarose have been used extensively for dielectric patterning of cells. In this study, we propose gelatin methacrylate (GelMA) as a promising hydrogel for use in cell dielectropatterning because of its biocompatibility and low viscosity. Compared to PEG hydrogels, GelMA hydrogels showed superior performance when making cell patterns for myoblast (C2C12) and endothelial (HUVEC) cells as well as in maintaining cell viability and growth. We also developed a simple and robust protocol for co-culture of these cells. Combined application of the GelMA hydrogels and the DEP technique is suitable for creating highly complex microscale tissues with important applications in fundamental cell biology and regenerative medicine in a rapid, accurate, and scalable manner. |
| 巻・号 | 12(16) |
| ページ | 2959-69 |
| 公開日 | 2012-8-21 |
| DOI | 10.1039/c2lc40213k |
| PMID | 22773042 |
| MeSH | Cell Line Cell Proliferation Cell Survival Coculture Techniques Electrophoresis, Agar Gel Gelatin / chemistry* Human Umbilical Vein Endothelial Cells Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry* Methacrylates / chemistry* Polyethylene Glycols / chemistry |
| IF | 6.774 |
| 引用数 | 113 |
| WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS NANOSCIENCE & NANOTECHNOLOGY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 39 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |