| RRC ID |
4395
|
| Author |
Pavanelli WR, Kaminami MS, Geres JR, Sano A, Ono MA, Camargo IC, Itano EN.
|
| Title |
Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis.
|
| Journal |
Mycopathologia
|
| Abstract |
Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (approximately 380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and production of IFNgamma (but not IL-4) were observed in "in vitro" spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM fraction (25 microg/protein = F-25 and 50 microg/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at 28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction has a protective effect in experimental paracoccidioidomycosis in BALB/c mice.
|
| Volume |
163(3)
|
| Pages |
117-28
|
| Published |
2007-3-1
|
| DOI |
10.1007/s11046-007-0095-4
|
| PMID |
17356792
|
| MeSH |
Animals
Antibodies, Fungal / blood
Antigens, Fungal / blood
Antigens, Fungal / immunology*
Antigens, Fungal / isolation & purification
Chromatography
Colony Count, Microbial
Cytokines / immunology
Female
Fungal Proteins / blood
Glycoproteins / blood
Granuloma / pathology
Immunization
Immunoglobulin G / blood
Liver / microbiology
Liver / pathology
Lung / microbiology
Lung / pathology
Mice
Mice, Inbred BALB C
Paracoccidioides / growth & development
Paracoccidioides / immunology*
Paracoccidioidomycosis / immunology*
Paracoccidioidomycosis / microbiology
Paracoccidioidomycosis / pathology
Spleen / cytology
Spleen / pathology
|
| IF |
2.452
|
| Times Cited |
7
|
|
WOS Category
|
MYCOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 41621 |
