RRC ID 4395
Author Pavanelli WR, Kaminami MS, Geres JR, Sano A, Ono MA, Camargo IC, Itano EN.
Title Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis.
Journal Mycopathologia
Abstract Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (approximately 380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and production of IFNgamma (but not IL-4) were observed in "in vitro" spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM fraction (25 microg/protein = F-25 and 50 microg/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at 28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction has a protective effect in experimental paracoccidioidomycosis in BALB/c mice.
Volume 163(3)
Pages 117-28
Published 2007-3
DOI 10.1007/s11046-007-0095-4
PMID 17356792
MeSH Animals Antibodies, Fungal / blood Antigens, Fungal / blood Antigens, Fungal / immunology* Antigens, Fungal / isolation & purification Chromatography Colony Count, Microbial Cytokines / immunology Female Fungal Proteins / blood Glycoproteins / blood Granuloma / pathology Immunization Immunoglobulin G / blood Liver / microbiology Liver / pathology Lung / microbiology Lung / pathology Mice Mice, Inbred BALB C Paracoccidioides / growth & development Paracoccidioides / immunology* Paracoccidioidomycosis / immunology* Paracoccidioidomycosis / microbiology Paracoccidioidomycosis / pathology Spleen / cytology Spleen / pathology
IF 2.278
Times Cited 7
Pathogenic microorganisms IFM 41621