RRC ID 43952
Author Koyama T, Nakajima C, Nishimoto S, Takami M, Woo JT, Yazawa K.
Title Suppressive effects of the leaf of Terminalia catappa L. on osteoclast differentiation in vitro and bone weight loss in vivo.
Journal J. Nutr. Sci. Vitaminol.
Abstract Oral administration of Terminalia catappa extract (TCE; 1,000 mg/kg) for 5 wk suppressed bone weight loss and trabecular bone loss in ovariectomized mice. An in vitro experiment showed that TCE (1.3-20 µg/mL) did not increase alkaline phosphatase activity, which would indicate osteoclast formation, in osteoblast-like 3T3-L1 cells. On the other hand, TCE (12.5 µg/mL) markedly decreased the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells, which would indicate osteoclast formation, in a co-culture system (bone marrow cells/osteoblastic UAMS-32 cells). A detailed analysis of the stages of osteoclast differentiation revealed that TCE mainly suppressed the differentiation of bone marrow mononuclear cells into osteoclast progenitor cells in the presence of M-CSF and TGF-β. An additional experiment using fractionated TCE revealed that the water-soluble fraction suppressed the bone weight loss in OVX-mice and osteoclast differentiation in vitro. Therefore, the suppressive effects of TCE on bone weight loss in mice might be due to the suppressive effects of highly polar components on the early stage of osteoclast differentiation.
Volume 58(2)
Pages 129-35
Published 2012
DOI 10.3177/jnsv.58.129
PII DN/JST.JSTAGE/jnsv/58.129
PMID 22790571
MeSH 3T3-L1 Cells Animals Bone and Bones / pathology Cell Differentiation / drug effects* Female Mice Organ Size / drug effects Osteoclasts / cytology* Osteoclasts / drug effects Osteoporosis / prevention & control* Ovariectomy Plant Extracts / administration & dosage* Plant Leaves / chemistry* Terminalia / chemistry*
IF 1.125
Times Cited 2
Human and Animal Cells