論文 - 詳細
| RRC ID | 43961 |
|---|---|
| 著者 | Miyoshi H, Yamaoka K, Urabe S, Kudo Y. |
| タイトル | ATP-induced currents carried through P2X7 receptor in rat myometrial cells. |
| ジャーナル | Reprod Sci |
| Abstract |
OBJECTIVE:The functional significance of purinergic P2 receptors in the myometrium is unclear. We previously reported the ATP-induced ionic currents in rat myometrial cells, causing uterine contractility. The aim of this study is to determine the main P2X receptors that carry the adenosine triphosphate (ATP)-induced currents. STUDY DESIGN:We cloned predominantly expressed P2X7 receptors from rat myometrium and transfected into cultured COS-7 cells. Reconstructed P2X7 receptor currents were characterized using the whole-cell patch clamp method. RESULTS:Extracellular ATP induced currents through P2X7 receptors with effective concentration (EC(50)) of 155 μmol/L, without desensitization. The myometrial P2X7 receptor was permeable to multiple monovalent cations with conductances ranked as K(+)>Cs(+)>Li(+)>Na(+). It was activated by P2X receptor agonists, with effectiveness ranked as 2',3'-O-(4-benzoylbenzoyl)-ATP (Bz-ATP)>ATP>αβ-methylene ATP (αβ-MeATP)>2-methylthio ATP (2-MeSATP)>uridine triphosphate (UTP)>guanosine triphosphate (GTP)>adenosine diphosphate (ADP). These currents were blocked by selective P2X7 receptor antagonists and extracellular Mg(2+). CONCLUSION:P2X7 receptors carry ATP-induced currents in rat myometrial cells. |
| 巻・号 | 19(12) |
| ページ | 1285-91 |
| 公開日 | 2012-12-1 |
| DOI | 10.1177/1933719112450333 |
| PII | 1933719112450333 |
| PMID | 22814097 |
| MeSH | Adenosine Triphosphate / agonists Adenosine Triphosphate / pharmacology* Animals COS Cells Chlorocebus aethiops Electric Conductivity Female Myometrium / chemistry Myometrium / physiology* Patch-Clamp Techniques Pregnancy Purinergic P2X Receptor Antagonists / pharmacology Rats Rats, Sprague-Dawley Receptors, Purinergic P2X4 / genetics Receptors, Purinergic P2X4 / physiology Receptors, Purinergic P2X7 / genetics Receptors, Purinergic P2X7 / physiology* Transfection |
| IF | 2.616 |
| 引用数 | 10 |
| WOS 分野 | OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |