| RRC ID |
4399
|
| 著者 |
Zeng JS, Fukushima K, Takizawa K, Zheng YC, Nishimura K, Gräser Y, De Hoog GS.
|
| タイトル |
Intraspecific diversity of species of the Pseudallescheria boydii complex.
|
| ジャーナル |
Med Mycol
|
| Abstract |
In order to establish intraspecific diversity of Pseudallescheria boydii and Scedosporium apiospermum, and to develop tools for their identification, variability within P. boydii and related species was investigated at different levels of diversity. Sequences of the D1/D2 region of large subunit (LSU) and of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) gene were analyzed for a set of 57 strains, as well as partial sequences of the elongation factor 1-alpha (EF 1-alpha). Incongruence among 3 locus lineages was detected by partition homogeneity test (PHT). The maximum parsimony (MP) tree of the combined sequence data set, with the exception of strain CBS 499.90, formed 3 clades with high bootstrap support, corresponding to previously described nuclear DNA (nDNA)/DNA reassociation groups. These groups are known to differ slightly in predilection and temperature relations. Using Structure software, population genetic analysis revealed 3 clusters within the complex on the basis of multi-locus genotype data. Strain distribution in the clusters was concordant with that in the 3 clades of combined multi-locus MP tree. Recombination among individuals of a clade in evolutional history was found in 2 of the 3 clades. There was population differentiation among the 3 clades. Restriction fragment length polymorphism (RFLP) analysis of the intergenic spacer (IGS) region of rDNA gene was added to further characterize subspecific entities. When the IGS regions of 22 strains were digested with the restriction endonucleases Hae III and Mbo I, seven and five distinct patterns were detected, respectively. This subtyping did not reveal any correspondence with geographic origin or clinical appearance. Though we need more evidence to locate the 3 clades of the P. boydii complex at species or population level, the sequence of the D1/D2 region is sufficiently variable for identification of taxa belonging to the P. boydii complex.
|
| 巻・号 |
45(6)
|
| ページ |
547-58
|
| 公開日 |
2007-9-1
|
| DOI |
10.1080/13693780701435341
|
| PII |
781445839
|
| PMID |
17710625
|
| MeSH |
DNA, Fungal / analysis
DNA, Fungal / isolation & purification
DNA, Ribosomal Spacer / analysis
Deoxyribonucleases, Type II Site-Specific / metabolism
Genetic Variation*
Genetics, Population
Humans
Molecular Sequence Data
Mycetoma / microbiology
Phylogeny
Polymorphism, Restriction Fragment Length
Pseudallescheria / classification*
Pseudallescheria / genetics
Recombination, Genetic
Scedosporium / classification*
Scedosporium / genetics
Sequence Analysis, DNA
Soil Microbiology
Species Specificity
|
| IF |
2.822
|
| 引用数 |
12
|
|
WOS 分野
|
VETERINARY SCIENCES
MYCOLOGY
INFECTIOUS DISEASES
|
| リソース情報 |
| 病原微生物 |
IFM 40911
IFM 41585
IFM 41595
IFM 41901
IFM 41911
IFM 41921
IFM 41923
IFM 46992
IFM 46993
IFM 47161
IFM 47302
IFM 48046
IFM 48461
IFM 49188
IFM 49724
IFM 49731
IFM 49770
IFM 50000
IFM 50231
IFM 50256
IFM 50907
IFM 50914
IFM 51940
IFM 52028
IFM 52858
IFM 52859
IFM 52860
IFM 52861
IFM 52862
IFM 52863
IFM 52864
IFM 52865
IFM 52873
IFM 52874
IFM 52875
IFM 52928
IFM 52929
IFM 52930 |
