論文 - 詳細
| RRC ID | 43990 |
|---|---|
| 著者 | Kakegawa T, Mochizuki N, Sadr N, Suzuki H, Fukuda J. |
| タイトル | Cell-adhesive and cell-repulsive zwitterionic oligopeptides for micropatterning and rapid electrochemical detachment of cells. |
| ジャーナル | Tissue Eng Part A |
| Abstract |
In this study, we describe the development of oligopeptide-modified cell culture surfaces from which adherent cells can be rapidly detached by application of an electrical stimulus. An oligopeptide, CGGGKEKEKEK, was designed with a terminal cysteine residue to mediate binding to a gold surface via a gold-thiolate bond. The peptide forms a self-assembled monolayer through the electrostatic force between the sequence of alternating charged glutamic acid (E) and lysine (K) residues. The dense and electrically neutral oligopeptide zwitterionic layer of the modified surface was resistant to nonspecific adsorption of proteins and adhesion of cells, while the surface was altered to cell adhesive by the addition of a second oligopeptide (CGGGKEKEKEKGRGDSP) containing the RGD cell adhesion motif. Application of a negative electrical potential to this gold surface cleaved the gold-thiolate bond, leading to desorption of the oligopeptide layer, and rapid (within 2 min) detachment of virtually all cells. This approach was applicable not only to detachment of cell sheets but also for transfer of cell micropatterns to a hydrogel. This electrochemical approach of cell detachment may be a useful tool for tissue-engineering applications. |
| 巻・号 | 19(1-2) |
| ページ | 290-8 |
| 公開日 | 2013-1-1 |
| DOI | 10.1089/ten.TEA.2011.0739 |
| PMID | 22853640 |
| PMC | PMC3530950 |
| MeSH | 3T3 Cells Amino Acid Transport Systems, Neutral / administration & dosage* Amino Acid Transport Systems, Neutral / chemistry Animals Cell Adhesion / physiology* Cell Adhesion / radiation effects* Cell Adhesion Molecules / administration & dosage* Cell Adhesion Molecules / chemistry Cell Separation / methods Electrochemistry / methods Electromagnetic Fields Mice Molecular Imprinting / methods* Oligopeptides / administration & dosage* Oligopeptides / chemistry Tissue Engineering / methods* |
| IF | 3.508 |
| 引用数 | 24 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 3T3 Swiss Albino(RCB1642) |