RRC ID 44012
Author Kato S, Kimura M, Kageyama K, Tanaka H, Miwa N.
Title Enhanced radiosensitization by liposome-encapsulated pimonidazole for anticancer effects on human melanoma cells.
Journal J Nanosci Nanotechnol
Abstract The nitroimidazole-related hypoxic radiosensitizer, pimonidazole (Pmz) was encapsulated in liposome composed of dipalmitoylphosphatidylcholine, cholesterol and dipalmitoylphosphatidylglycerol (molar ratio = 1:1:0.2; diameter = 112.9 nm), and the radiosensitization was evaluated in human melanoma cells HMV-II. Cell proliferation was examined by WST-8 assay after X-ray irradiation in the presence of liposomal Pmz or free-Pmz under hypoxic conditions. On 7th day after X-ray irradiation of 5 Gy, cell proliferation decreased more markedly in the administration of liposomal Pmz than free-Pmz at equivalent Pmz doses. Chromatin fragmentation or nuclear condensation was observed in liposomal Pmz-treated HMV-II cells. Radiosensitization was enhanced dose-dependently along with Pmz amounts of 250-2000 microM contained in liposomal Pmz. Intracellular uptake was more abundant for liposomal Pmz for 60-240 min than for free-Pmz. Thus liposomal Pmz has a potential to overcome radiation resistance in hypoxia, owing to enhanced intracellular uptake by melanoma cells.
Volume 12(6)
Pages 4472-7
Published 2012-6
DOI 10.1166/jnn.2012.6180
PMID 22905487
MeSH Cell Line Cell Survival / drug effects Cell Survival / radiation effects Humans Liposomes / chemistry* Materials Testing Melanoma / pathology* Melanoma / physiopathology Melanoma / radiotherapy* Nanocapsules / administration & dosage Nanocapsules / chemistry* Nitroimidazoles / administration & dosage* Nitroimidazoles / chemistry Radiation Tolerance / drug effects* Radiation-Sensitizing Agents / administration & dosage* Radiation-Sensitizing Agents / chemistry
IF 1.354
Times Cited 6
WOS Category PHYSICS, APPLIED PHYSICS, CONDENSED MATTER MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY
Resource
Human and Animal Cells