| RRC ID |
44012
|
| 著者 |
Kato S, Kimura M, Kageyama K, Tanaka H, Miwa N.
|
| タイトル |
Enhanced radiosensitization by liposome-encapsulated pimonidazole for anticancer effects on human melanoma cells.
|
| ジャーナル |
J Nanosci Nanotechnol
|
| Abstract |
The nitroimidazole-related hypoxic radiosensitizer, pimonidazole (Pmz) was encapsulated in liposome composed of dipalmitoylphosphatidylcholine, cholesterol and dipalmitoylphosphatidylglycerol (molar ratio = 1:1:0.2; diameter = 112.9 nm), and the radiosensitization was evaluated in human melanoma cells HMV-II. Cell proliferation was examined by WST-8 assay after X-ray irradiation in the presence of liposomal Pmz or free-Pmz under hypoxic conditions. On 7th day after X-ray irradiation of 5 Gy, cell proliferation decreased more markedly in the administration of liposomal Pmz than free-Pmz at equivalent Pmz doses. Chromatin fragmentation or nuclear condensation was observed in liposomal Pmz-treated HMV-II cells. Radiosensitization was enhanced dose-dependently along with Pmz amounts of 250-2000 microM contained in liposomal Pmz. Intracellular uptake was more abundant for liposomal Pmz for 60-240 min than for free-Pmz. Thus liposomal Pmz has a potential to overcome radiation resistance in hypoxia, owing to enhanced intracellular uptake by melanoma cells.
|
| 巻・号 |
12(6)
|
| ページ |
4472-7
|
| 公開日 |
2012-6-1
|
| DOI |
10.1166/jnn.2012.6180
|
| PMID |
22905487
|
| MeSH |
Cell Line
Cell Survival / drug effects
Cell Survival / radiation effects
Humans
Liposomes / chemistry*
Materials Testing
Melanoma / pathology*
Melanoma / physiopathology
Melanoma / radiotherapy*
Nanocapsules / administration & dosage
Nanocapsules / chemistry*
Nitroimidazoles / administration & dosage*
Nitroimidazoles / chemistry
Radiation Tolerance / drug effects*
Radiation-Sensitizing Agents / administration & dosage*
Radiation-Sensitizing Agents / chemistry
|
| IF |
1.134
|
| 引用数 |
8
|
|
WOS 分野
|
PHYSICS, APPLIED
PHYSICS, CONDENSED MATTER
MATERIALS SCIENCE, MULTIDISCIPLINARY
CHEMISTRY, MULTIDISCIPLINARY
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HMV-II(RCB0777) |
