Reference - Detail
| RRC ID | 44014 |
|---|---|
| Author | Zhu Q, Shibata T, Kabashima T, Kai M. |
| Title | Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-mediated specific delivery of siRNA. |
| Journal | Eur J Med Chem |
| Abstract |
Targeted delivery is a promising way to improve the safety and efficiency of siRNA delivery. We show that a DNA aptamer could be used to deliver siRNA into CD4(+) T cells specifically. The DNA aptamer was obtained from the conversion of a reported RNA aptamer that binds to CD4 protein on the surface of T cells. It was covalently conjugated to the sense strand of the siRNA targeting HIV-1 protease (HIV-PR). The resulting DNA aptamer-siRNA chimera could specifically enter into CD4(+) T cells and efficiently knock down the expression of exogenous HIV-PR gene. This study provides the first evidence that the DNA aptamer with intrinsic stability has a greater potential to be used for siRNA delivery. |
| Volume | 56 |
| Pages | 396-9 |
| Published | 2012-10-1 |
| DOI | 10.1016/j.ejmech.2012.07.045 |
| PII | S0223-5234(12)00471-0 |
| PMID | 22907035 |
| MeSH | Aptamers, Nucleotide / metabolism* CD4-Positive T-Lymphocytes / metabolism* Drug Delivery Systems* Gene Expression Regulation, Enzymologic / drug effects* Gene Expression Regulation, Enzymologic / genetics HIV Protease / genetics HIV Protease / metabolism* Protease Inhibitors / chemical synthesis Protease Inhibitors / chemistry Protease Inhibitors / pharmacology* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA, Small Interfering / pharmacology* |
| IF | 5.573 |
| Times Cited | 31 |
| WOS Category | CHEMISTRY, MEDICINAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |