| RRC ID |
44014
|
| Author |
Zhu Q, Shibata T, Kabashima T, Kai M.
|
| Title |
Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-mediated specific delivery of siRNA.
|
| Journal |
Eur J Med Chem
|
| Abstract |
Targeted delivery is a promising way to improve the safety and efficiency of siRNA delivery. We show that a DNA aptamer could be used to deliver siRNA into CD4(+) T cells specifically. The DNA aptamer was obtained from the conversion of a reported RNA aptamer that binds to CD4 protein on the surface of T cells. It was covalently conjugated to the sense strand of the siRNA targeting HIV-1 protease (HIV-PR). The resulting DNA aptamer-siRNA chimera could specifically enter into CD4(+) T cells and efficiently knock down the expression of exogenous HIV-PR gene. This study provides the first evidence that the DNA aptamer with intrinsic stability has a greater potential to be used for siRNA delivery.
|
| Volume |
56
|
| Pages |
396-9
|
| Published |
2012-10-1
|
| DOI |
10.1016/j.ejmech.2012.07.045
|
| PII |
S0223-5234(12)00471-0
|
| PMID |
22907035
|
| MeSH |
Aptamers, Nucleotide / metabolism*
CD4-Positive T-Lymphocytes / metabolism*
Drug Delivery Systems*
Gene Expression Regulation, Enzymologic / drug effects*
Gene Expression Regulation, Enzymologic / genetics
HIV Protease / genetics
HIV Protease / metabolism*
Protease Inhibitors / chemical synthesis
Protease Inhibitors / chemistry
Protease Inhibitors / pharmacology*
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
RNA, Small Interfering / pharmacology*
|
| IF |
5.573
|
| Times Cited |
31
|
|
WOS Category
|
CHEMISTRY, MEDICINAL
|
| Resource |
| Human and Animal Cells |
|
