論文 - 詳細
| RRC ID | 44020 |
|---|---|
| 著者 | Kitamoto N, Oka T, Katayama K, Li TC, Takeda N, Kato Y, Miyoshi T, Tanaka T. |
| タイトル | Novel monoclonal antibodies broadly reactive to human recombinant sapovirus-like particles. |
| ジャーナル | Microbiol Immunol |
| Abstract |
Sapovirus (SaV), a member of the family Caliciviridae, is an important cause of acute epidemic gastroenteritis in humans. Human SaV is genetically and antigenically diverse and can be classified into four genogroups (GI, GII, GIV, and GV) and 16 genotypes (7 GI [GI.1-7], 7 GII, [GII.1-7], 1 GIV and 1 GV), based on capsid sequence similarities. Monoclonal antibodies (MAbs) are powerful tools for examining viruses and proteins. PAI myeloma cells were fused with spleen cells from mice immunized with a single type of recombinant human SaV virus-like particles (VLPs) (GI.1, GI.5, GI.6, GII.3, GIV, or GV). Sixty-five hybrid clones producing MAbs were obtained. Twenty-four MAbs were characterized by ELISA, according to their cross-reactivity to each VLP (GI.1, GI.5, GI.6, GII.2, GII.3, GII.4, GII.7, GIV, and GV). The MAbs were classified by this method into: (i) MAbs broadly cross-reactive to all GI, GII, GIV and GV strains; (ii) those reactive in a genogroup-specific; and (iii) those reactive in a genotype-specific manner. Further analysis of three broadly cross-reactive MAbs with a competitive ELISA demonstrated that at least two different common epitopes are located on the capsid protein of human SaVs in the four genogroups. The MAbs generated and characterized in this study will be useful tools for further study of the antigenic and structural topography of the human SaV virion and for developing new diagnostic assays for human SaV. |
| 巻・号 | 56(11) |
| ページ | 760-70 |
| 公開日 | 2012-11-1 |
| DOI | 10.1111/j.1348-0421.2012.00499.x |
| PMID | 22924483 |
| MeSH | Animals Antibodies, Monoclonal / immunology* Antibodies, Monoclonal / isolation & purification Antibodies, Viral / immunology* Antibodies, Viral / isolation & purification Capsid Proteins / immunology Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes / immunology Genotype Humans Mice Mice, Inbred BALB C Sapovirus / classification Sapovirus / genetics Sapovirus / immunology* |
| IF | 1.566 |
| 引用数 | 4 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |