| RRC ID |
44027
|
| Author |
Takarada T, Kodama A, Hotta S, Mieda M, Shimba S, Hinoi E, Yoneda Y.
|
| Title |
Clock genes influence gene expression in growth plate and endochondral ossification in mice.
|
| Journal |
J Biol Chem
|
| Abstract |
We have previously shown transient promotion by parathyroid hormone of Period-1 (Per1) expression in cultured chondrocytes. Here we show the modulation by clock genes of chondrogenic differentiation through gene transactivation of the master regulator of chondrogenesis Indian hedgehog (IHH) in chondrocytes of the growth plate. Several clock genes were expressed with oscillatory rhythmicity in cultured chondrocytes and rib growth plate in mice, whereas chondrogenesis was markedly inhibited in stable transfectants of Per1 in chondrocytic ATDC5 cells and in rib growth plate chondrocytes from mice deficient of brain and muscle aryl hydrocarbon receptor nuclear translocator-like (BMAL1). Ihh promoter activity was regulated by different clock gene products, with clear circadian rhythmicity in expression profiles of Ihh in the growth plate. In BMAL1-null mice, a predominant decrease was seen in Ihh expression in the growth plate with a smaller body size than in wild-type mice. BMAL1 deficit led to disruption of the rhythmic expression profiles of both Per1 and Ihh in the growth plate. A clear rhythmicity was seen with Ihh expression in ATDC5 cells exposed to dexamethasone. In young mice defective of BMAL1 exclusively in chondrocytes, similar abnormalities were found in bone growth and Ihh expression. These results suggest that endochondral ossification is under the regulation of particular clock gene products expressed in chondrocytes during postnatal skeletogenesis through a mechanism relevant to the rhythmic Ihh expression.
|
| Volume |
287(43)
|
| Pages |
36081-95
|
| Published |
2012-10-19
|
| DOI |
10.1074/jbc.M112.408963
|
| PII |
S0021-9258(20)62584-4
|
| PMID |
22936800
|
| PMC |
PMC3476276
|
| MeSH |
ARNTL Transcription Factors / genetics
ARNTL Transcription Factors / metabolism*
Animals
Anti-Inflammatory Agents / pharmacology
Biological Clocks / physiology*
Cell Line
Chondrocytes / cytology
Chondrocytes / metabolism*
Chondrogenesis / drug effects
Chondrogenesis / physiology
Dexamethasone / pharmacology
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology*
Growth Plate / cytology
Growth Plate / metabolism*
Hedgehog Proteins / biosynthesis*
Hedgehog Proteins / genetics
Mice
Mice, Knockout
Osteogenesis / drug effects
Osteogenesis / physiology*
Period Circadian Proteins / genetics
Period Circadian Proteins / metabolism
Promoter Regions, Genetic / physiology
|
| IF |
4.238
|
| Times Cited |
57
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
ATDC5(RCB0565) |
