RRC ID 44027
Author Takarada T, Kodama A, Hotta S, Mieda M, Shimba S, Hinoi E, Yoneda Y.
Title Clock genes influence gene expression in growth plate and endochondral ossification in mice.
Journal J Biol Chem
Abstract We have previously shown transient promotion by parathyroid hormone of Period-1 (Per1) expression in cultured chondrocytes. Here we show the modulation by clock genes of chondrogenic differentiation through gene transactivation of the master regulator of chondrogenesis Indian hedgehog (IHH) in chondrocytes of the growth plate. Several clock genes were expressed with oscillatory rhythmicity in cultured chondrocytes and rib growth plate in mice, whereas chondrogenesis was markedly inhibited in stable transfectants of Per1 in chondrocytic ATDC5 cells and in rib growth plate chondrocytes from mice deficient of brain and muscle aryl hydrocarbon receptor nuclear translocator-like (BMAL1). Ihh promoter activity was regulated by different clock gene products, with clear circadian rhythmicity in expression profiles of Ihh in the growth plate. In BMAL1-null mice, a predominant decrease was seen in Ihh expression in the growth plate with a smaller body size than in wild-type mice. BMAL1 deficit led to disruption of the rhythmic expression profiles of both Per1 and Ihh in the growth plate. A clear rhythmicity was seen with Ihh expression in ATDC5 cells exposed to dexamethasone. In young mice defective of BMAL1 exclusively in chondrocytes, similar abnormalities were found in bone growth and Ihh expression. These results suggest that endochondral ossification is under the regulation of particular clock gene products expressed in chondrocytes during postnatal skeletogenesis through a mechanism relevant to the rhythmic Ihh expression.
Volume 287(43)
Pages 36081-95
Published 2012-10-19
DOI 10.1074/jbc.M112.408963
PII M112.408963
PMID 22936800
PMC PMC3476276
MeSH ARNTL Transcription Factors / genetics ARNTL Transcription Factors / metabolism* Animals Anti-Inflammatory Agents / pharmacology Biological Clocks / physiology* Cell Line Chondrocytes / cytology Chondrocytes / metabolism* Chondrogenesis / drug effects Chondrogenesis / physiology Dexamethasone / pharmacology Gene Expression Regulation / drug effects Gene Expression Regulation / physiology* Growth Plate / cytology Growth Plate / metabolism* Hedgehog Proteins / biosynthesis* Hedgehog Proteins / genetics Mice Mice, Knockout Osteogenesis / drug effects Osteogenesis / physiology* Period Circadian Proteins / genetics Period Circadian Proteins / metabolism Promoter Regions, Genetic / physiology
IF 4.106
Times Cited 57
Human and Animal Cells