論文 - 詳細
| RRC ID | 44038 |
|---|---|
| 著者 | Tabata K, Hamano A, Akihisa T, Suzuki T. |
| タイトル | Kuguaglycoside C, a constituent of Momordica charantia, induces caspase-independent cell death of neuroblastoma cells. |
| ジャーナル | Cancer Sci |
| Abstract |
Kuguaglycoside C is a triterpene glycoside isolated from the leaves of Momordica charantia, and the biological effects of this compound remain almost unknown. We investigated the anti-cancer effect of kuguaglycoside C against human neuroblastoma IMR-32 cells. In the MTT assay, kuguaglycoside C induced significant cytotoxicity against the IMR-32 cells (IC(50) : 12.6 μM) after 48 h treatment. Although examination by Hoechst 33342 staining revealed that kuguaglycoside C induced nuclear shrinkage at a high concentration (100 μM), no apoptotic bodies were observed on flow cytometry. No activation of caspase-3 or caspase-9 was observed at the effective concentration (30 μM) of kuguaglycoside C. On the other hand, the substance significantly decreased the expression of survivin and cleaved poly (ADP-ribose) polymerase (PARP). Kuguaglycoside C also significantly increased the expression and cleavage of apoptosis-inducing factor (AIF). Moreover, kuguaglycoside C was found to induce caspase-independent DNA cleavage in the dual-fluorescence apoptosis detection assay. These results suggest that kuguaglycoside C induces caspase-independent cell death, and is involved, at least in part, in the mechanism underlying cell necroptosis. |
| 巻・号 | 103(12) |
| ページ | 2153-8 |
| 公開日 | 2012-12-1 |
| DOI | 10.1111/cas.12021 |
| PMID | 22957888 |
| PMC | PMC7659232 |
| MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Apoptosis Caspase 3 / metabolism* Caspase 9 / metabolism* Cell Death* DNA Cleavage Flow Cytometry Glycosides / chemistry Glycosides / pharmacology* Humans Momordica charantia / chemistry* Neuroblastoma Poly(ADP-ribose) Polymerases / genetics Poly(ADP-ribose) Polymerases / metabolism Saponins / chemistry* Saponins / pharmacology Tumor Cells, Cultured |
| IF | 4.966 |
| 引用数 | 17 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IMR-32(RCB1895) |