Reference - Detail
| RRC ID | 44040 |
|---|---|
| Author | Watari A, Yagi K, Kondoh M. |
| Title | A simple reporter assay for screening claudin-4 modulators. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Claudin-4, a member of a tetra-transmembrane protein family that comprises 27 members, is a key functional and structural component of the tight junction-seal in mucosal epithelium. Modulation of the claudin-4-barrier for drug absorption is now of research interest. Disruption of the claudin-4-seal occurs during inflammation. Therefore, claudin-4 modulators (repressors and inducers) are promising candidates for drug development. However, claudin-4 modulators have never been fully developed. Here, we attempted to design a screening system for claudin-4 modulators by using a reporter assay. We prepared a plasmid vector coding a claudin-4 promoter-driven luciferase gene and established stable reporter gene-expressing cells. We identified thiabendazole, carotene and curcumin as claudin-4 inducers, and potassium carbonate as a claudin-4 repressor by using the reporter cells. They also increased or decreased, respectively, the integrity of the tight junction-seal in Caco-2 cells. This simple reporter system will be a powerful tool for the development of claudin-4 modulators. |
| Volume | 426(4) |
| Pages | 454-60 |
| Published | 2012-10-5 |
| DOI | 10.1016/j.bbrc.2012.08.083 |
| PII | S0006-291X(12)01602-6 |
| PMID | 22960168 |
| MeSH | Cell Line, Tumor Claudin-4 / agonists* Claudin-4 / antagonists & inhibitors* Drug Evaluation, Preclinical / methods Gene Expression / drug effects Genes, Reporter / drug effects* Humans Luciferases / genetics Plasmids / genetics Promoter Regions, Genetic / drug effects |
| IF | 2.985 |
| Times Cited | 8 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MCF7(RCB1904) |