| RRC ID |
44040
|
| Author |
Watari A, Yagi K, Kondoh M.
|
| Title |
A simple reporter assay for screening claudin-4 modulators.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Claudin-4, a member of a tetra-transmembrane protein family that comprises 27 members, is a key functional and structural component of the tight junction-seal in mucosal epithelium. Modulation of the claudin-4-barrier for drug absorption is now of research interest. Disruption of the claudin-4-seal occurs during inflammation. Therefore, claudin-4 modulators (repressors and inducers) are promising candidates for drug development. However, claudin-4 modulators have never been fully developed. Here, we attempted to design a screening system for claudin-4 modulators by using a reporter assay. We prepared a plasmid vector coding a claudin-4 promoter-driven luciferase gene and established stable reporter gene-expressing cells. We identified thiabendazole, carotene and curcumin as claudin-4 inducers, and potassium carbonate as a claudin-4 repressor by using the reporter cells. They also increased or decreased, respectively, the integrity of the tight junction-seal in Caco-2 cells. This simple reporter system will be a powerful tool for the development of claudin-4 modulators.
|
| Volume |
426(4)
|
| Pages |
454-60
|
| Published |
2012-10-5
|
| DOI |
10.1016/j.bbrc.2012.08.083
|
| PII |
S0006-291X(12)01602-6
|
| PMID |
22960168
|
| MeSH |
Cell Line, Tumor
Claudin-4 / agonists*
Claudin-4 / antagonists & inhibitors*
Drug Evaluation, Preclinical / methods
Gene Expression / drug effects
Genes, Reporter / drug effects*
Humans
Luciferases / genetics
Plasmids / genetics
Promoter Regions, Genetic / drug effects
|
| IF |
2.985
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MCF7(RCB1904) |
