論文 - 詳細
| RRC ID | 44045 |
|---|---|
| 著者 | Imanishi M, Yamamoto K, Yamada H, Hirose Y, Okamura H, Futaki S. |
| タイトル | Construction of a rhythm transfer system that mimics the cellular clock. |
| ジャーナル | ACS Chem Biol |
| Abstract |
Creation of an artificial oscillating gene expression system is one of the most challenging issues in synthetic biology. Here, we constructed a simple system to manipulate gene expression patterns to be circadian, reflecting the intrinsic cellular clock, by fusing a core clock protein, BMAL1 or CLOCK, with a zinc finger-type DNA binding domain. Circadian rhythmic gene expression was induced only when the target gene contained zinc finger-binding sequences. To our knowledge, this simple approach is the first to manipulate gene expression patterns into circadian rhythms and would be applicable to various endogenous genes. |
| 巻・号 | 7(11) |
| ページ | 1817-21 |
| 公開日 | 2012-11-16 |
| DOI | 10.1021/cb300432s |
| PMID | 22967180 |
| MeSH | ARNTL Transcription Factors / genetics* Animals CLOCK Proteins / genetics* Circadian Rhythm* Genes, Reporter Mice NIH 3T3 Cells Promoter Regions, Genetic Recombinant Fusion Proteins / genetics Transcriptional Activation* Vascular Endothelial Growth Factor A / genetics Zinc Fingers* |
| IF | 4.434 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |