RRC ID 44045
Author Imanishi M, Yamamoto K, Yamada H, Hirose Y, Okamura H, Futaki S.
Title Construction of a rhythm transfer system that mimics the cellular clock.
Journal ACS Chem. Biol.
Abstract Creation of an artificial oscillating gene expression system is one of the most challenging issues in synthetic biology. Here, we constructed a simple system to manipulate gene expression patterns to be circadian, reflecting the intrinsic cellular clock, by fusing a core clock protein, BMAL1 or CLOCK, with a zinc finger-type DNA binding domain. Circadian rhythmic gene expression was induced only when the target gene contained zinc finger-binding sequences. To our knowledge, this simple approach is the first to manipulate gene expression patterns into circadian rhythms and would be applicable to various endogenous genes.
Volume 7(11)
Pages 1817-21
Published 2012-11-16
DOI 10.1021/cb300432s
PMID 22967180
MeSH ARNTL Transcription Factors / genetics* Animals CLOCK Proteins / genetics* Circadian Rhythm* Genes, Reporter Mice NIH 3T3 Cells Promoter Regions, Genetic Recombinant Fusion Proteins / genetics Transcriptional Activation* Vascular Endothelial Growth Factor A / genetics Zinc Fingers*
IF 4.374
Times Cited 2
Human and Animal Cells