| RRC ID |
44045
|
| 著者 |
Imanishi M, Yamamoto K, Yamada H, Hirose Y, Okamura H, Futaki S.
|
| タイトル |
Construction of a rhythm transfer system that mimics the cellular clock.
|
| ジャーナル |
ACS Chem Biol
|
| Abstract |
Creation of an artificial oscillating gene expression system is one of the most challenging issues in synthetic biology. Here, we constructed a simple system to manipulate gene expression patterns to be circadian, reflecting the intrinsic cellular clock, by fusing a core clock protein, BMAL1 or CLOCK, with a zinc finger-type DNA binding domain. Circadian rhythmic gene expression was induced only when the target gene contained zinc finger-binding sequences. To our knowledge, this simple approach is the first to manipulate gene expression patterns into circadian rhythms and would be applicable to various endogenous genes.
|
| 巻・号 |
7(11)
|
| ページ |
1817-21
|
| 公開日 |
2012-11-16
|
| DOI |
10.1021/cb300432s
|
| PMID |
22967180
|
| MeSH |
ARNTL Transcription Factors / genetics*
Animals
CLOCK Proteins / genetics*
Circadian Rhythm*
Genes, Reporter
Mice
NIH 3T3 Cells
Promoter Regions, Genetic
Recombinant Fusion Proteins / genetics
Transcriptional Activation*
Vascular Endothelial Growth Factor A / genetics
Zinc Fingers*
|
| IF |
4.434
|
| 引用数 |
3
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
