RRC ID 44053
著者 Kubo T, Yanagihara K, Sato Y, Morita Y, Seyama T.
タイトル Enhancement of gene silencing effect and membrane permeability by Peptide-conjugated 27-nucleotide small interfering RNA.
ジャーナル Molecules
Abstract Two different sizes of siRNAs, of which one type was 21-nucleotide (nt) siRNA containing 2-nt dangling ends and the other type was 27-nt siRNA with blunt ends, were conjugated with a nuclear export signal peptide of HIV-1 Rev at the 5'-sense end. Processing by Dicer enzyme, cell membrane permeability, and RNAi efficiency of the peptide-conjugated siRNAs were examined. Dicer cleaved the peptide-conjugated 27-nt siRNA leading to the release of 21-nt siRNA, whereas the peptide-conjugated 21-nt siRNA was not cleaved. High membrane permeability and cytoplasmic localization was found in the conjugates. Moreover, the peptide-conjugated 27-nt siRNA showed increased potency of RNAi in comparison with the nonmodified 21-nt and 27-nt siRNAs, whereas the peptide-conjugated 21-nt siRNA showed decreased RNAi efficacy. This potent RNAi efficacy is probably owing to acceleration of RISC through recognition by Dicer, as well as to the improvement of cell membrane permeability and intracellular accumulation.
巻・号 17(9)
ページ 11089-102
公開日 2012-9-14
DOI 10.3390/molecules170911089
PII molecules170911089
PMID 22983148
PMC PMC6268710
MeSH Animals Cell Line, Tumor Cell Membrane Permeability HeLa Cells Humans Luciferases, Renilla / genetics* Peptides / chemistry RNA Interference* RNA, Small Interfering* / chemistry RNA, Small Interfering* / genetics RNA, Small Interfering* / metabolism Renilla / genetics rev Gene Products, Human Immunodeficiency Virus / chemistry
IF 3.267
引用数 4
WOS 分野 CHEMISTRY, ORGANIC
リソース情報
ヒト・動物細胞 HeLa(RCB0007)