| RRC ID |
44065
|
| 著者 |
Hatoh T, Maeda T, Takeuchi K, Ogikubo O, Uchiyama S, Otsuka T, Ohkubo I, Ogita H.
|
| タイトル |
Domain 5 of high molecular weight kininogen inhibits collagen-mediated cancer cell adhesion and invasion in association with α-actinin-4.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
High molecular weight kininogen (HK) is a plasma glycoprotein with multiple functions, including the regulation of coagulation. We previously demonstrated that domain 5 (D5(H)), a functional domain of HK, and its derived peptides played an important role in the vitronectin-mediated suppression of cancer cell adhesion and invasion. However, the underlying mechanisms of the D5(H)-mediated suppressive effects remain to be elucidated. Here, we showed that D5(H) and its derivatives inhibited the collagen-mediated cell adhesion and invasion of human osteosarcoma MG63 cells. Using purified D5(H) fused to glutathione-S-transferase (GST) and D5(H)-derived peptides for column chromatography, an actin-binding protein, α-actinin-4, was identified as a binding protein of D5(H) with high-affinity for P-5m, a core octapeptide of D5(H). Immunofluorescence microscopy demonstrated that D5(H) co-localized with α-actinin-4 inside MG63 cells. In addition, exogenous GST-D5(H) added to the culture media was transported into MG63 cells, although GST alone as a control was not. As α-actinin-4 regulates actin polymerization necessary for cell adhesion and is related to the integrin-dependent attachment of cells to the extracellular matrix, our results suggest that D5(H) may modulate cell adhesion and invasion together with actinin-4.
|
| 巻・号 |
427(3)
|
| ページ |
497-502
|
| 公開日 |
2012-10-26
|
| DOI |
10.1016/j.bbrc.2012.09.079
|
| PII |
S0006-291X(12)01826-8
|
| PMID |
23000411
|
| MeSH |
Actinin / metabolism*
Amino Acid Sequence
Cell Adhesion
Cell Line, Tumor
Collagen / metabolism
Humans
Kininogen, High-Molecular-Weight / genetics
Kininogen, High-Molecular-Weight / metabolism*
Kininogen, High-Molecular-Weight / pharmacology
Molecular Sequence Data
Neoplasm Invasiveness
Neoplasms / metabolism
Neoplasms / pathology*
Peptide Fragments / genetics
Peptide Fragments / metabolism
Peptide Fragments / pharmacology
|
| IF |
2.985
|
| 引用数 |
4
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MG-63(RCB1890) |
