Reference - Detail
|Author||An SH, Matsumoto T, Miyajima H, Nakahira A, Kim KH, Imazato S.|
|Title||Porous zirconia/hydroxyapatite scaffolds for bone reconstruction.|
OBJECTIVE:Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect.
METHODS:To address the hypothesis that using a ZrO(2)/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO(2)/HAp composite scaffolds with different ZrO(2)/HAp ratios, and evaluated their characteristics. In addition, porous ZrO(2)/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material.
RESULTS:The porosity of a ZrO(2)/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8MPa when the ZrO(2) content increased from 50 to 100wt%. The cell adhesion and proliferation in the ZrO(2)/HAp scaffold was greatly improved when compared to the scaffold made with ZrO(2) alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO(2)/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material.
SIGNIFICANCE:We thus showed that a porous ZrO(2)/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects.
|MeSH||3T3 Cells Animals Biocompatible Materials / chemical synthesis Biocompatible Materials / chemistry* Biomechanical Phenomena Bone Diseases / surgery* Bone Marrow Cells / physiology Bone Regeneration / physiology Cell Adhesion / physiology Cell Proliferation Cell Survival / physiology Collagen Type I / analysis Compressive Strength Crystallography, X-Ray Durapatite / chemical synthesis Durapatite / chemistry* Male Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Osteoblasts / physiology Osteogenesis / physiology Osteopontin / analysis Parietal Bone / surgery Porosity Rats Reconstructive Surgical Procedures / methods* Stromal Cells / physiology Tissue Engineering / methods Tissue Scaffolds / chemistry* Zirconium / chemistry*|
|WOS Category||DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS|
|Human and Animal Cells|