論文 - 詳細
| RRC ID | 44078 |
|---|---|
| 著者 | Suzuki J, Hamada E, Shodai T, Kamoshida G, Kudo S, Itoh S, Koike J, Nagata K, Irimura T, Tsuji T. |
| タイトル | Cytokine secretion from human monocytes potentiated by P-selectin-mediated cell adhesion. |
| ジャーナル | Int Arch Allergy Immunol |
| Abstract |
BACKGROUND/AIM:P-selectin is a carbohydrate-recognizing cell adhesion molecule expressed on activated platelets and endothelial cells. It plays a crucial role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. Cell adhesion mediated by P-selectin induces leukocyte activation, such as the generation of reactive oxygen species and the expression of blood coagulation factors. We assessed how P-selectin-mediated cell adhesion affects cytokine secretion from monocytes. METHODS:Human peripheral blood monocytes were cultured in a plate that had been coated with P-selectin purified from human platelets, and cytokines released in the culture supernatant from monocytes were determined by ELISA. RESULTS:The secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, IL-12 and macrophage inflammatory protein-1β increased 3- to 10-fold in response to P-selectin compared with unstimulated monocytes. We next examined the effects of cytokine treatment of monocytes on their susceptibility to P-selectin. The secretion of TNF-α from monocytes in response to P-selectin was increased when monocytes were preincubated with granulocyte/macrophage colony-stimulating factor, monocyte chemotactic protein-1 or interferon-γ (IFN-γ); IFN-γ was the most effective in potentiating TNF-α secretion from monocytes. CONCLUSION:These results suggest that the interaction of monocytes with P-selectin plays an important role not only in their trafficking but also in the regulation of cytokine production by these cells. |
| 巻・号 | 160(2) |
| ページ | 152-60 |
| 公開日 | 2013-1-1 |
| DOI | 10.1159/000339857 |
| PII | 000339857 |
| PMID | 23018521 |
| MeSH | Cell Adhesion Cytokines / metabolism* Humans Monocytes / cytology* Monocytes / immunology Monocytes / metabolism* P-Selectin / metabolism* |
| IF | 2.917 |
| 引用数 | 18 |
| WOS 分野 | ALLERGY IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |