Reference - Detail
| RRC ID | 44091 |
|---|---|
| Author | Koizumi S, Ohno S, Otsuka F. |
| Title | A versatile transfection assay system to evaluate the biological effects of diverse industrial chemicals. |
| Journal | Biol Pharm Bull |
| Abstract |
Gene expression processes are now recognized as important targets of the toxic effects exerted by industrial chemicals. The transient transfection assay is a powerful tool to evaluate such effects. Thus, we developed a versatile assay system by constructing a basic reporter plasmid in which the regulatory DNA sequence to be studied can easily be substituted. To verify the performance of this system, reporter plasmids carrying any of the three distinct regulatory sequences, estrogen responsive element (ERE), glucocorticoid responsive element (GRE) and xenobiotic responsive element (XRE) were constructed. After transfection of human cells, these plasmids successfully expressed the relevant reporter genes in response to specific inducers, β-estradiol, dexamethasone and 3-methylcholanthrene, respectively. Several industrial chemicals were assayed using these reporter plasmids, and the ability of p-dimethylaminoazobenzene to elevate GRE- and XRE-mediated transcription was detected. α-Naphthylamine and o-tolidine were also observed to increase the XRE-mediated response. The transfection assay system established here will be useful to evaluate the effects of a wide variety of industrial chemicals. |
| Volume | 35(10) |
| Pages | 1691-6 |
| Published | 2012-1-1 |
| DOI | 10.1248/bpb.b12-00172 |
| PII | DN/JST.JSTAGE/bpb/b12-00172 |
| PMID | 23037159 |
| MeSH | Biological Assay / methods* Cell Line, Tumor Gene Expression Regulation / drug effects* Genes, Reporter / genetics Humans Plasmids Response Elements / genetics* Simplexvirus Thymidine Kinase / genetics Transfection Viral Proteins / genetics Xenobiotics / toxicity* |
| IF | 1.863 |
| Times Cited | 0 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |