論文 - 詳細
| RRC ID | 44100 |
|---|---|
| 著者 | Raveendran S, Poulose AC, Yoshida Y, Maekawa T, Kumar DS. |
| タイトル | Bacterial exopolysaccharide based nanoparticles for sustained drug delivery, cancer chemotherapy and bioimaging. |
| ジャーナル | Carbohydr Polym |
| Abstract |
Introduction of a novel biocompatible, stable, biomaterial for drug delivery application remains always challenging. In the present study, we report the synthesis of an extremophilic bacterial sulfated polysaccharide based nanoparticle as a stable biocompatible material for drug delivery, evaluation of anticancer efficacy and bioimaging. Mauran (MR), the sulfated exopolysaccharide extracted from a moderately halophilic bacterium, Halomonas maura was used for the synthesis of nanoparticles along with chitosan (CH). MR/CH nanoparticles were synthesized by simple polyelectrolyte complexation of anionic MR and cationic CH. The MR/CH hybrid nanoparticles formed were ranging between 30 and 200 nm in diameter with an overall positive zeta potential of 27.5±5 mV and was found to be stable under storage in solution for at least 8 weeks. In vitro drug release studies showed a sustained and prolonged delivery of 5-fluorouracil (5FU) for 10-12 days from MR/CH nanoparticles under three different pHs of 4.5, 6.9 and 7.4 respectively. Cytotoxicity assay revealed that MR/CH nanoparticles were non-cytotoxic towards normal cells and toxic to cancer cells. Also, 5FU loaded MR/CH nanoparticles were found more effective than free 5FU in its sustained and controlled manner of killing breast adenocarcinoma cells. Fluorescein isothiocyanate (FITC) labeled MR/CH nanoparticles were used for cell binding and uptake studies; thereby demonstrating the application of dye tagged MR/CH nanoparticles for safe and nontoxic mode of live cellular imaging. We report the introduction of an extremophilic bacterial polysaccharide, MR, for the first time as a novel biocompatible and stable biomaterial to the world of nanotechnology, pharmaceutics and biomedical technology. |
| 巻・号 | 91(1) |
| ページ | 22-32 |
| 公開日 | 2013-1-2 |
| DOI | 10.1016/j.carbpol.2012.07.079 |
| PII | S0144-8617(12)00752-7 |
| PMID | 23044101 |
| MeSH | Animals Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Biocompatible Materials / chemistry* Cell Line, Tumor Chemistry, Pharmaceutical Drug Carriers / chemistry* Fluorouracil / chemistry Fluorouracil / pharmacology Halomonas / chemistry Halomonas / ultrastructure Humans Kinetics Mice Microscopy, Electron, Scanning Molecular Imaging* Nanoparticles / chemistry* Polysaccharides, Bacterial / chemistry* |
| IF | 7.182 |
| 引用数 | 42 |
| WOS 分野 | CHEMISTRY, APPLIED POLYMER SCIENCE CHEMISTRY, ORGANIC |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) L929 |