| RRC ID |
44100
|
| 著者 |
Raveendran S, Poulose AC, Yoshida Y, Maekawa T, Kumar DS.
|
| タイトル |
Bacterial exopolysaccharide based nanoparticles for sustained drug delivery, cancer chemotherapy and bioimaging.
|
| ジャーナル |
Carbohydr Polym
|
| Abstract |
Introduction of a novel biocompatible, stable, biomaterial for drug delivery application remains always challenging. In the present study, we report the synthesis of an extremophilic bacterial sulfated polysaccharide based nanoparticle as a stable biocompatible material for drug delivery, evaluation of anticancer efficacy and bioimaging. Mauran (MR), the sulfated exopolysaccharide extracted from a moderately halophilic bacterium, Halomonas maura was used for the synthesis of nanoparticles along with chitosan (CH). MR/CH nanoparticles were synthesized by simple polyelectrolyte complexation of anionic MR and cationic CH. The MR/CH hybrid nanoparticles formed were ranging between 30 and 200 nm in diameter with an overall positive zeta potential of 27.5±5 mV and was found to be stable under storage in solution for at least 8 weeks. In vitro drug release studies showed a sustained and prolonged delivery of 5-fluorouracil (5FU) for 10-12 days from MR/CH nanoparticles under three different pHs of 4.5, 6.9 and 7.4 respectively. Cytotoxicity assay revealed that MR/CH nanoparticles were non-cytotoxic towards normal cells and toxic to cancer cells. Also, 5FU loaded MR/CH nanoparticles were found more effective than free 5FU in its sustained and controlled manner of killing breast adenocarcinoma cells. Fluorescein isothiocyanate (FITC) labeled MR/CH nanoparticles were used for cell binding and uptake studies; thereby demonstrating the application of dye tagged MR/CH nanoparticles for safe and nontoxic mode of live cellular imaging. We report the introduction of an extremophilic bacterial polysaccharide, MR, for the first time as a novel biocompatible and stable biomaterial to the world of nanotechnology, pharmaceutics and biomedical technology.
|
| 巻・号 |
91(1)
|
| ページ |
22-32
|
| 公開日 |
2013-1-2
|
| DOI |
10.1016/j.carbpol.2012.07.079
|
| PII |
S0144-8617(12)00752-7
|
| PMID |
23044101
|
| MeSH |
Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Biocompatible Materials / chemistry*
Cell Line, Tumor
Chemistry, Pharmaceutical
Drug Carriers / chemistry*
Fluorouracil / chemistry
Fluorouracil / pharmacology
Halomonas / chemistry
Halomonas / ultrastructure
Humans
Kinetics
Mice
Microscopy, Electron, Scanning
Molecular Imaging*
Nanoparticles / chemistry*
Polysaccharides, Bacterial / chemistry*
|
| IF |
7.182
|
| 引用数 |
42
|
|
WOS 分野
|
CHEMISTRY, APPLIED
POLYMER SCIENCE
CHEMISTRY, ORGANIC
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904)
L929 |
