RRC ID 44104
著者 Inoue H, Miyazaki Y, Kikuchi K, Yoshida N, Ide F, Ohmori Y, Tomomura A, Sakashita H, Kusama K.
タイトル Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
ジャーナル J Oral Sci
Abstract Human podoplanin is a type-1 transmembrane sialomucin-like glycoprotein that is involved in cell migration, tumor cell invasion and metastasis. Our recent study of oral squamous cell carcinoma (OSCC) demonstrated that the degree of immunohistochemical expression of podoplanin was correlated with the severity of epithelial dysplasia and significantly associated with a poor pathologic grade of differentiation. Furthermore, it has been reported that Src directly associates with the epidermal growth factor receptor (EGFR) in OSCC cells upon stimulation with EGF and phosphorylates Crk-associated substrate (Cas), podoplanin acting downstream of Src and Cas to promote cell migration. However, the molecular function of podoplanin remains unclear. In this study we performed real-time RT-PCR, Western blotting and scratch assay using OSCC cell lines in order to clarify the molecular biological function of podoplanin expression associated with various growth factors including EGF and with the Src-Cas signaling pathway. Podoplanin was found to have a marked influence on cancer cell migration and the expression of matrix metalloprotease-9 (MMP-9) in the oral cavity upon stimulation with EGF. Podoplanin promotes oral cancer cell migration, and the EGF-Src-Cas pathway is one of the possible mechanisms responsible for progression of cancer in the oral cavity.
巻・号 54(3)
ページ 241-50
公開日 2012-9-1
DOI 10.2334/josnusd.54.241
PII DN/JST.JSTAGE/josnusd/54.241
PMID 23047035
MeSH Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cell Movement* / drug effects Crk-Associated Substrate Protein Epidermal Growth Factor / physiology Humans MAP Kinase Signaling System* Matrix Metalloproteinase 9 / metabolism Membrane Glycoproteins / pharmacology Membrane Glycoproteins / physiology* Mouth Neoplasms / metabolism* Mouth Neoplasms / pathology Neoplasm Invasiveness Neoplasm Proteins / pharmacology Neoplasm Proteins / physiology* Reverse Transcriptase Polymerase Chain Reaction Tumor Microenvironment
IF 1.2
引用数 23
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902)