| RRC ID |
44108
|
| 著者 |
Kamoshida G, Matsuda A, Miura R, Takashima Y, Katsura A, Tsuji T.
|
| タイトル |
Potentiation of tumor cell invasion by co-culture with monocytes accompanying enhanced production of matrix metalloproteinase and fibronectin.
|
| ジャーナル |
Clin Exp Metastasis
|
| Abstract |
Macrophages are a major population of immune cells, and those that infiltrate into tumor tissues and affect the malignant behavior of tumor cells are called tumor-associated macrophages (TAMs). We previously reported that human peripheral blood monocytes could be induced in vitro to differentiate into TAM-like cells by co-culture with tumor cells. In the present study, we characterized changes in the invasive phenotype of tumor cells after co-culture with monocytes, and found that MKN1 gastric carcinoma cells acquired higher invasive potential into Matrigel-reconstituted basement membranes, accompanied by enhanced production of matrix metalloproteinase (MMP)-9. The increased invasiveness was inhibited in the presence of an arginyl-glycyl-aspartic acid peptide, suggesting that the process is dependent on the integrin-extracellular matrix interaction. We also found that these cells secreted fibronectin into the culture medium and expressed α5 integrin on their surface at higher levels after the co-culture with monocytes for 5 days. The conditioned medium of monocytes also potentiated MKN1 cell invasion; however, the potentiation was lowered by the depletion of tumor necrosis factor (TNF)-α from the conditioned medium with an antibody-protein G-Sepharose conjugate. In addition, the treatment of MKN1 cells with TNF-α promoted invasion of these cells, as well as secretion of MMP-9 and fibronectin. These results suggest that TNF-α secreted from monocytes is, at least in part, involved in the changes in invasive phenotype of tumor cells during co-culture with monocytes.
|
| 巻・号 |
30(3)
|
| ページ |
289-97
|
| 公開日 |
2013-3-1
|
| DOI |
10.1007/s10585-012-9536-7
|
| PMID |
23053742
|
| MeSH |
Blotting, Western
Cell Line, Tumor
Coculture Techniques
Culture Media, Conditioned
Fibronectins / biosynthesis*
Flow Cytometry
Humans
Matrix Metalloproteinases / biosynthesis*
Monocytes / pathology*
Neoplasm Invasiveness*
Stomach Neoplasms / enzymology
Stomach Neoplasms / metabolism
Stomach Neoplasms / pathology*
Tumor Necrosis Factor-alpha / metabolism
|
| IF |
3.027
|
| 引用数 |
17
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003) |
