論文 - 詳細
| RRC ID | 44108 |
|---|---|
| 著者 | Kamoshida G, Matsuda A, Miura R, Takashima Y, Katsura A, Tsuji T. |
| タイトル | Potentiation of tumor cell invasion by co-culture with monocytes accompanying enhanced production of matrix metalloproteinase and fibronectin. |
| ジャーナル | Clin Exp Metastasis |
| Abstract |
Macrophages are a major population of immune cells, and those that infiltrate into tumor tissues and affect the malignant behavior of tumor cells are called tumor-associated macrophages (TAMs). We previously reported that human peripheral blood monocytes could be induced in vitro to differentiate into TAM-like cells by co-culture with tumor cells. In the present study, we characterized changes in the invasive phenotype of tumor cells after co-culture with monocytes, and found that MKN1 gastric carcinoma cells acquired higher invasive potential into Matrigel-reconstituted basement membranes, accompanied by enhanced production of matrix metalloproteinase (MMP)-9. The increased invasiveness was inhibited in the presence of an arginyl-glycyl-aspartic acid peptide, suggesting that the process is dependent on the integrin-extracellular matrix interaction. We also found that these cells secreted fibronectin into the culture medium and expressed α5 integrin on their surface at higher levels after the co-culture with monocytes for 5 days. The conditioned medium of monocytes also potentiated MKN1 cell invasion; however, the potentiation was lowered by the depletion of tumor necrosis factor (TNF)-α from the conditioned medium with an antibody-protein G-Sepharose conjugate. In addition, the treatment of MKN1 cells with TNF-α promoted invasion of these cells, as well as secretion of MMP-9 and fibronectin. These results suggest that TNF-α secreted from monocytes is, at least in part, involved in the changes in invasive phenotype of tumor cells during co-culture with monocytes. |
| 巻・号 | 30(3) |
| ページ | 289-97 |
| 公開日 | 2013-3-1 |
| DOI | 10.1007/s10585-012-9536-7 |
| PMID | 23053742 |
| MeSH | Blotting, Western Cell Line, Tumor Coculture Techniques Culture Media, Conditioned Fibronectins / biosynthesis* Flow Cytometry Humans Matrix Metalloproteinases / biosynthesis* Monocytes / pathology* Neoplasm Invasiveness* Stomach Neoplasms / enzymology Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Tumor Necrosis Factor-alpha / metabolism |
| IF | 3.027 |
| 引用数 | 17 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN1(RCB1003) |