Reference - Detail
| RRC ID | 44109 |
|---|---|
| Author | Bae JY, Mie M, Kobatake E. |
| Title | Targeted gene delivery via PEI complexed with an antibody. |
| Journal | Appl Biochem Biotechnol |
| Abstract |
Stable carriers are required for gene delivery. The use of polyethyleneimine (PEI) has been researched extensively; however, it is not suitable for targeted gene delivery to specific cells. To provide a targeting ability of the specific antibody to PEI, two repeats of Fc-binding domain of protein G (C2) were utilized. The constructed protein containing C2 could bind to a specific antibody and form a larger complex with plasmid DNA/PEI complex. The specific antibody to cell surface protein was bound to the complex through the use of C2 domain, and it was added to cells. As the result, the efficient expression of delivered reporter gene could be realized. |
| Volume | 168(8) |
| Pages | 2184-90 |
| Published | 2012-12-1 |
| DOI | 10.1007/s12010-012-9928-9 |
| PMID | 23054824 |
| MeSH | Antibodies / chemistry* Antibodies / genetics Antibodies / metabolism Antibody Specificity Bacterial Proteins / chemistry DNA / genetics DNA / metabolism HeLa Cells Humans Polyethyleneimine / chemistry* Protein Structure, Tertiary Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Transfection / methods* |
| IF | 2.277 |
| Times Cited | 2 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |