| RRC ID |
44127
|
| 著者 |
Morita Y, Mukai T, Ju Y, Watanabe S.
|
| タイトル |
Evaluation of stem cell-to-tenocyte differentiation by atomic force microscopy to measure cellular elastic moduli.
|
| ジャーナル |
Cell Biochem Biophys
|
| Abstract |
In the present study, we evaluated whether stem cell-to-tenocyte differentiation could be evaluated via measurement of the mechanical properties of the cell. We used mechanical uniaxial cyclic stretching to induce the differentiation of human bone marrow mesenchymal stem cells into tenocytes. The cells were subjected to cyclic elongation of 10 or 15 % at a cyclic frequency of 1 Hz for 24 or 48 h, and differentiation was assessed by real-time PCR (rtPCR) determination of messenger RNA expression levels for four commonly used markers of stem cell-to-tenocyte differentiation: type I collagen, type III collagen, tenascin-C, and scleraxis. The rtPCR results showed that cells subjected to 10 % cyclic elongation for 24 or 48 h differentiated into tenocytes. Atomic force microscopy (AFM) was then used to measure the force curves around the cell nuclei, and the AFM data were used to calculate the elastic moduli of the cell surfaces. The elastic modulus values of the control (non-stretched) cells differed significantly from those of cells stretched at 10 % for 24 or 48 h (P < 0.01). Confocal fluorescence microscopic observations of actin stress fibers suggested that the change in elastic modulus was ascribable to the development of the cellular cytoskeleton during the differentiation process. Therefore, we conclude that the atomic force microscopic measurement of the elastic modulus of the cell surface can be used to evaluate stem cell-to-tenocyte differentiation.
|
| 巻・号 |
66(1)
|
| ページ |
73-80
|
| 公開日 |
2013-5-1
|
| DOI |
10.1007/s12013-012-9455-x
|
| PMID |
23090789
|
| MeSH |
Actin Cytoskeleton
Biomarkers / analysis
Biomechanical Phenomena
Cell Differentiation*
Cell Line
Cell Nucleus / genetics
Cell Nucleus / metabolism
Cell Shape
Collagen Type I / genetics
Collagen Type I / metabolism
Collagen Type III / genetics
Collagen Type III / metabolism
Elastic Modulus*
Humans
Microscopy, Atomic Force / methods*
RNA, Messenger
Stem Cells / cytology*
Stem Cells / metabolism
Stress Fibers / metabolism
Tenascin / metabolism
Time Factors
|
| IF |
2.073
|
| 引用数 |
18
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
UE6E7T-3(RCB2154) |
