論文 - 詳細
| RRC ID | 44128 |
|---|---|
| 著者 | Saito S, Tsugeno M, Koto D, Mori Y, Yoshioka Y, Nohara S, Murase K. |
| タイトル | Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages. |
| ジャーナル | Int J Nanomedicine |
| Abstract |
PURPOSE:Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites. MATERIALS AND METHODS:We tested four SPIO and ultrasmall superparamagnetic iron oxide (USPIO), alkali-treated dextran magnetite (ATDM) with particle sizes of 28 and 74 nm, and carboxymethyl dextran magnetite (CMDM) with particle sizes of 28 and 72 nm. Mouse macrophage RAW264 cells were incubated with SPIOs and USPIOs, and the labeling efficiency of the cells was determined by the percentage of Berlin blue-stained cells and by measuring T(2) relaxation times with 11.7-T MRI. We used trypan blue staining to measure cell viability. RESULTS:Analysis of the properties of the nanoparticles revealed that ATDM-coated 74 nm particles have a lower T(2) relaxation time than the others, translating into a higher ability of MRI negative contrast agent. Among the other three candidates, CMDM-coated particles showed the highest T(2) relaxation time once internalized by macrophages. Regarding labeling efficiency, ATDM coating resulted in a cellular uptake higher than CMDM coating, independent of nanoparticle size. None of these particle formulations affected macrophage viability. CONCLUSION:This study suggests that coating is more critical than size to optimize the SPIO labeling of macrophages. Among the formulations tested in this study, the best MRI contrast and labeling efficiency are expected with ATDM-coated 74 nm nanoparticles. |
| 巻・号 | 7 |
| ページ | 5415-21 |
| 公開日 | 2012-1-1 |
| DOI | 10.2147/IJN.S33709 |
| PII | ijn-7-5415 |
| PMID | 23091384 |
| PMC | PMC3474462 |
| MeSH | Coated Materials, Biocompatible / chemistry* Contrast Media / chemistry Crystallization / methods Dextrans / chemistry* Diffusion Macromolecular Substances / chemistry Macrophages / chemistry* Macrophages / cytology* Magnetic Resonance Imaging / methods* Magnetite Nanoparticles / chemistry* Materials Testing Molecular Conformation Particle Size Surface Properties |
| IF | 5.115 |
| 引用数 | 36 |
| WOS 分野 | PHARMACOLOGY & PHARMACY NANOSCIENCE & NANOTECHNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |