Reference - Detail
| RRC ID | 44132 |
|---|---|
| Author | Kusano-Arai O, Iwanari H, Mochizuki Y, Nakata H, Kodama T, Kitoh T, Hamakubo T. |
| Title | Evaluation of the asparagine synthetase level in leukemia cells by monoclonal antibodies. |
| Journal | Hybridoma (Larchmt) |
| Abstract |
L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells. |
| Volume | 31(5) |
| Pages | 325-32 |
| Published | 2012-10-1 |
| DOI | 10.1089/hyb.2012.0014 |
| PMID | 23098298 |
| MeSH | Animals Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / immunology* Antibodies, Monoclonal / isolation & purification Aspartate-Ammonia Ligase / genetics Aspartate-Ammonia Ligase / immunology Aspartate-Ammonia Ligase / metabolism* Baculoviridae / genetics Blotting, Western Cell Line, Tumor Chlorocebus aethiops Enzyme-Linked Immunosorbent Assay Flow Cytometry Genetic Vectors Humans Immunization Mice Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / immunology Recombinant Fusion Proteins / metabolism Transfection |
| Times Cited | 2 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | K562 MOLT-4(RCB0206) |