| RRC ID |
44132
|
| Author |
Kusano-Arai O, Iwanari H, Mochizuki Y, Nakata H, Kodama T, Kitoh T, Hamakubo T.
|
| Title |
Evaluation of the asparagine synthetase level in leukemia cells by monoclonal antibodies.
|
| Journal |
Hybridoma (Larchmt)
|
| Abstract |
L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells.
|
| Volume |
31(5)
|
| Pages |
325-32
|
| Published |
2012-10-1
|
| DOI |
10.1089/hyb.2012.0014
|
| PMID |
23098298
|
| MeSH |
Animals
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology*
Antibodies, Monoclonal / isolation & purification
Aspartate-Ammonia Ligase / genetics
Aspartate-Ammonia Ligase / immunology
Aspartate-Ammonia Ligase / metabolism*
Baculoviridae / genetics
Blotting, Western
Cell Line, Tumor
Chlorocebus aethiops
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Genetic Vectors
Humans
Immunization
Mice
Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology
Recombinant Fusion Proteins / metabolism
Transfection
|
| Times Cited |
2
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMICAL RESEARCH METHODS
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
K562
MOLT-4(RCB0206) |
