Reference - Detail
| RRC ID | 44143 |
|---|---|
| Author | Lambrechts D, Roeffaers M, Kerckhofs G, Roberts SJ, Hofkens J, Van de Putte T, Van Oosterwyck H, Schrooten J. |
| Title | Fluorescent oxygen sensitive microbead incorporation for measuring oxygen tension in cell aggregates. |
| Journal | Biomaterials |
| Abstract |
Molecular oxygen is a main regulator of various cell functions. Imaging methods designed as screening tools for fast, in situ, 3D and non-interfering measurement of oxygen tension in the cellular microenvironment would serve great purpose in identifying and monitoring this vital and pivotal signalling molecule. We describe the use of dual luminophore oxygen sensitive microbeads to measure absolute oxygen concentrations in cellular aggregates. Stable microbead integration, a prerequisite for their practical application, was ensured by a site-specific delivery method that is based on the interactions between streptavidin and biotin. The spatial stability introduced by this method allowed for long term measurements of oxygen tension without interfering with the cell aggregation process. By making multiple calibration experiments we further demonstrated the potential of these sensors to measure local oxygen tension in optically dense cellular environments. |
| Volume | 34(4) |
| Pages | 922-9 |
| Published | 2013-1-1 |
| DOI | 10.1016/j.biomaterials.2012.10.019 |
| PII | S0142-9612(12)01140-4 |
| PMID | 23122803 |
| MeSH | Animals Biological Assay / methods* Cell Aggregation / physiology Cell Line Chondrocytes / cytology Chondrocytes / metabolism* Mice Microspheres Molecular Imaging / methods* Oximetry / methods* Oxygen / analysis* Oxygen Consumption / physiology* Spectrometry, Fluorescence / methods* |
| IF | 10.317 |
| Times Cited | 20 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |