RRC ID |
44157
|
著者 |
Kobayashi T, Yukigai J, Ueda K, Machida K, Masutani M, Nishino Y, Miyazawa A, Imataka H.
|
タイトル |
Purification and visualization of encephalomyocarditisvirus synthesized by an in vitro protein expression system derived from mammalian cell extract.
|
ジャーナル |
Biotechnol Lett
|
Abstract |
Virus particles are promising vehicles and templates for vaccination, drug delivery and material sciences. Although infectious picornaviruses can be synthesized from genomic or synthetic RNA by cell-free protein expression systems derived from mammalian cell extract, there has been no direct evidence that authentic viral particles are indeed synthesized in the absence of living cells. We purified encephalomyocarditis virus (EMCV) synthesized by a HeLa cell extract-derived, cell-free protein expression system, and visualized the viral particles by transmission electron-microscopy. The in vitro-synthesized EMCV particles were indistinguishable from the in vivo-synthesized particles. Our results validate the use of the cell-free technique for the synthesis of EMCV particles.
|
巻・号 |
35(3)
|
ページ |
309-14
|
公開日 |
2013-3-1
|
DOI |
10.1007/s10529-012-1086-1
|
PMID |
23143176
|
MeSH |
Biotechnology / methods*
Cell Extracts / isolation & purification*
Cell-Free System
Encephalomyocarditis virus / isolation & purification*
Encephalomyocarditis virus / ultrastructure
HeLa Cells
Humans
Microscopy, Electron, Transmission
Viral Proteins / biosynthesis*
Virion / isolation & purification
Virion / ultrastructure
|
IF |
1.977
|
引用数 |
5
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
BHK-21
HeLa S3 |