RRC ID 44157
著者 Kobayashi T, Yukigai J, Ueda K, Machida K, Masutani M, Nishino Y, Miyazawa A, Imataka H.
タイトル Purification and visualization of encephalomyocarditisvirus synthesized by an in vitro protein expression system derived from mammalian cell extract.
ジャーナル Biotechnol Lett
Abstract Virus particles are promising vehicles and templates for vaccination, drug delivery and material sciences. Although infectious picornaviruses can be synthesized from genomic or synthetic RNA by cell-free protein expression systems derived from mammalian cell extract, there has been no direct evidence that authentic viral particles are indeed synthesized in the absence of living cells. We purified encephalomyocarditis virus (EMCV) synthesized by a HeLa cell extract-derived, cell-free protein expression system, and visualized the viral particles by transmission electron-microscopy. The in vitro-synthesized EMCV particles were indistinguishable from the in vivo-synthesized particles. Our results validate the use of the cell-free technique for the synthesis of EMCV particles.
巻・号 35(3)
ページ 309-14
公開日 2013-3-1
DOI 10.1007/s10529-012-1086-1
PMID 23143176
MeSH Biotechnology / methods* Cell Extracts / isolation & purification* Cell-Free System Encephalomyocarditis virus / isolation & purification* Encephalomyocarditis virus / ultrastructure HeLa Cells Humans Microscopy, Electron, Transmission Viral Proteins / biosynthesis* Virion / isolation & purification Virion / ultrastructure
IF 1.977
引用数 5
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 BHK-21 HeLa S3