論文 - 詳細
| RRC ID | 44164 |
|---|---|
| 著者 | Sasaki H, Karasawa K, Hironaka K, Tahara K, Tozuka Y, Takeuchi H. |
| タイトル | Retinal drug delivery using eyedrop preparations of poly-L-lysine-modified liposomes. |
| ジャーナル | Eur J Pharm Biopharm |
| Abstract |
The purpose of this study was to develop surface-modified liposomes that enhance the efficiency of eye drop drug delivery to the retina. Various molecular weights and concentrations of the water-soluble cationic polymer poly-L-lysine (PLL) were used to modify the surface of submicronized (100 nm) liposomes. Physicochemical properties of surface-modified liposomes were determined in vitro, and the efficiency of drug delivery to the retina was investigated in vivo. Using coumarin-6 as a model drug and fluorescent marker, we show that liposome surface modification by PLL dramatically increased delivery to mouse retina segments after eye drop administration. However, when PLL of high molecular weight (>30,000) was used at higher concentrations (>0.05%), aggregation of surface-modified liposomes increased particle size and hampered distribution to inner ocular tissues. As a result, the efficiency of drug delivery of these aggregated surface-modified liposomes was the same as unmodified liposomes. The optimal molecular weight and concentration of PLL in drug-delivering liposomes were 15,000-30,000 and 0.005%, respectively. Under these conditions, PLL-modified liposomes were not cytotoxic in corneal or conjunctival cells. In conclusion, surface-modified liposomes have great potential as effective retinal drug delivery carriers in eye drop formulations. |
| 巻・号 | 83(3) |
| ページ | 364-9 |
| 公開日 | 2013-4-1 |
| DOI | 10.1016/j.ejpb.2012.10.014 |
| PII | S0939-6411(12)00340-2 |
| PMID | 23153668 |
| MeSH | Animals Drug Delivery Systems* Liposomes* Male Mice Ophthalmic Solutions* Polylysine / chemistry* Retina* |
| IF | 4.604 |
| 引用数 | 34 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |