論文 - 詳細
| RRC ID | 44172 |
|---|---|
| 著者 | Yang JJ, Liu JF, Kurokawa T, Kitada K, Gong JP. |
| タイトル | Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells. |
| ジャーナル | J Tissue Eng Regen Med |
| Abstract |
Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro. |
| 巻・号 | 9(4) |
| ページ | 375-88 |
| 公開日 | 2015-4-1 |
| DOI | 10.1002/term.1640 |
| PMID | 23166055 |
| MeSH | Animals Antigens, Differentiation / biosynthesis* Cell Proliferation* Coated Materials, Biocompatible / chemical synthesis Coated Materials, Biocompatible / chemistry* Gelatin / chemistry Hydrogels / chemical synthesis Hydrogels / chemistry* Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Mice Polystyrenes / chemistry Tissue Scaffolds / chemistry* |
| IF | 3.078 |
| 引用数 | 10 |
| WOS 分野 | ENGINEERING, BIOMEDICAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |