| RRC ID |
44172
|
| 著者 |
Yang JJ, Liu JF, Kurokawa T, Kitada K, Gong JP.
|
| タイトル |
Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.
|
| ジャーナル |
J Tissue Eng Regen Med
|
| Abstract |
Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.
|
| 巻・号 |
9(4)
|
| ページ |
375-88
|
| 公開日 |
2015-4-1
|
| DOI |
10.1002/term.1640
|
| PMID |
23166055
|
| MeSH |
Animals
Antigens, Differentiation / biosynthesis*
Cell Proliferation*
Coated Materials, Biocompatible / chemical synthesis
Coated Materials, Biocompatible / chemistry*
Gelatin / chemistry
Hydrogels / chemical synthesis
Hydrogels / chemistry*
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Mice
Polystyrenes / chemistry
Tissue Scaffolds / chemistry*
|
| IF |
3.078
|
| 引用数 |
10
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001) |
