| RRC ID |
44173
|
| 著者 |
Motomura W, Yoshizaki T, Takahashi N, Kumei S, Mizukami Y, Jang SJ, Kohgo Y.
|
| タイトル |
Analysis of vanin-1 upregulation and lipid accumulation in hepatocytes in response to a high-fat diet and free fatty acids.
|
| ジャーナル |
J Clin Biochem Nutr
|
| Abstract |
High-fat diet is one of the causes of nonalcoholic fatty liver disease. We have previously demonstrated that high-fat diet induces upregulation of adipose differentiation-related protein mRNA expression accompanied by lipid droplet formation in mouse liver. Vanin-1 is a ubiquitous epithelial ectoenzyme that has pantetheinase activity and produces cysteamine, a potent endogenous antioxidant. In the present study, we analyzed the expression of hepatic vanin-1 mRNA following the administration of a high-fat diet in mice as well as free fatty acids in hepatocyte cultures and speculated its possible mechanism. Vanin-1 mRNA levels in the livers of mice were upregulated within a day of the high-fat diet, even before the expression of adipose differentiation-related protein mRNA and lipid accumulation. An in vitro analysis using HuH-7 cells revealed a significant upregulation of vanin-1 mRNA by as low as 0.01 mM oleic acid; however, lipid accumulation in hepatocytes was not affected at this concentration. Furthermore, vanin-1 mRNA was differentially upregulated by various free fatty acids irrespective of the grade of lipid accumulation. These findings indicate that the upregulation of vanin-1 precedes lipid accumulation and is differentially mediated by various types of free fatty acids in the model, presenting vanin-1 as a novel player in the pathogenesis of nonalcoholic fatty liver disease.
|
| 巻・号 |
51(3)
|
| ページ |
163-9
|
| 公開日 |
2012-11-1
|
| DOI |
10.3164/jcbn.12-06
|
| PII |
jcbn12-06
|
| PMID |
23170042
|
| PMC |
PMC3491239
|
| IF |
2.405
|
| 引用数 |
6
|
|
WOS 分野
|
NUTRITION & DIETETICS
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7 |
