Reference - Detail
| RRC ID | 44197 |
|---|---|
| Author | Itoh T, Miyake Y, Onda A, Kubo J, Ando M, Tsukamasa Y, Takahata M. |
| Title | Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells. |
| Journal | Microbiologyopen |
| Abstract |
The objective of this study was to investigate the immunomodulatory effects of heat-killed Enterococcus faecalis TH10 (hk-TH10) and its signal transduction on murine macrophage RAW264 cells. RAW264 cells produced nitric oxide (NO) following hk-TH10 treatment. In order to investigate the mechanisms underlying hk-TH10-stimulated NO production, we further measured NO production in RAW264 cells treated with Toll-like receptor (TLR) 4 inhibitor peptide, NF-κB inhibitor, TLR1-siRNA, TLR2-siRNA, and TLR-6 siRNA. Furthermore, the activation of TLR2-TLR1/6 pathway molecules was analyzed by Western blotting. The result of this study showed that hk-TH10 stimulates NO in RAW264 cells through the activation of the TLR2-TLR1/6 pathway. From our findings, we can conclude that hk-TH10 isolated from a traditional side-dish fermented food (tempeh) may facilitate host immunomodulation. |
| Volume | 1(4) |
| Pages | 373-80 |
| Published | 2012-12-1 |
| DOI | 10.1002/mbo3.41 |
| PMID | 23233442 |
| PMC | PMC3535383 |
| MeSH | Animals Blotting, Western Cell Line Enterococcus faecalis / immunology* Macrophage Activation / immunology Macrophages / immunology* Macrophages / microbiology* Mice NF-kappa B / immunology Nitric Oxide / analysis Nitric Oxide / immunology RNA, Small Interfering / administration & dosage Signal Transduction Toll-Like Receptors / immunology |
| IF | 3.142 |
| Times Cited | 5 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 19 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |